Targeting Glioma Cancer Cells with Fluorescent Nanodiamonds via Integrin Receptors

Part of the Methods in Pharmacology and Toxicology book series (MIPT)


Glioblastomas, aggressive and highly vascularized brain tumors, overexpress αvβ3 integrins, which are widely exploited for cancer diagnostics and therapy. Proteins and peptides containing the RGD sequence bind αvβ3 integrins. Here, we describe detailed protocols for preparation and testing of fluorescent nanodiamonds coated with a biocompatible N-(2-hydroxypropyl)methacrylamide copolymer shell. When the surface of these particles was modified with a cyclic RGD peptide, they selectively targeted integrin αvβ3 receptors on U-87 MG glioblastoma cells with high internalization efficacy. The modified particles enabled background-free near-infrared imaging of cells, showed excellent colloidal stability in culture media, and exhibited negligible cytotoxicity.


Background-free imaging Fluorescent nanodiamond Glioma RGD peptide Targeting αvβ3 integrin 



This work was supported by the Czech Science Foundation project Nr. 16-16336S (to J.N. and P.C.) and Nr. 16-03156S (to M.H.). Irradiations were performed at the CANAM infrastructure of the NPI CAS Rez supported through MŠMT project No. LM2011019. Imaging was performed on confocal microscope supported by Project NPU I, LO 1302 from the Ministry of Education, Youth and Sports of the Czech Republic.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Institute of Organic Chemistry and Biochemistry of the CASPrague 6Czech Republic
  2. 2.First Faculty of MedicineCharles UniversityPrague 2Czech Republic
  3. 3.Nuclear Physics Institute of the CAS250 68 Rez near PragueCzech Republic
  4. 4.Institute of Macromolecular Chemistry of the CASPrague 6Czech Republic
  5. 5.Department of Genetics and Microbiology, Faculty of ScienceCharles UniversityPrague 2Czech Republic

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