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Easy and Rapid Methods for Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells and Human Umbilical Wharton’s Jelly–Derived Mesenchymal Stem Cells

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Stem Cells and Lineage Commitment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2736))

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Abstract

These protocols describe modified methods that use Ficoll-Paque density gradient for umbilical cord blood–derived mesenchymal stem cells and explant method for Wharton’s jelly–derived mesenchymal stem cells. The Ficoll-Paque density gradient method allows to obtain mesenchymal stem cells while eliminating monocytic cells. In this method, precoating the cell culture flasks with fetal bovine serum helps remove the monocytic cells and instruct more pure mesenchymal stem cells. On the other hand, the explant method for Wharton’s jelly–derived mesenchymal stem cell is user-friendly and cost-effective than enzymatic methods. In this chapter, we provide a collection of protocols to obtain mesenchymal stem cells from human umbilical cord blood and Wharton’s jelly.

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Author information

Authors and Affiliations

  1. Istanbul University, Istanbul Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey

    Figen Abatay Sel, Ayse Erol, Mediha Suleymanoglu & Fatma Savran Oguz

  2. Department of Medical Biology, Istanbul University, Institute of Graduate Studies in Health Science, Istanbul, Turkey

    Figen Abatay Sel

  3. Istanbul University, Istanbul Faculty of Medicine, Department of Physiology, Istanbul, Turkey

    Durdane Serap Kuruca

  4. Istanbul Atlas University, Faculty of Medicine, Department of Physiology, Istanbul, Turkey

    Durdane Serap Kuruca

Authors
  1. Figen Abatay Sel
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  2. Ayse Erol
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  3. Mediha Suleymanoglu
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  4. Durdane Serap Kuruca
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  5. Fatma Savran Oguz
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Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Abatay Sel, F., Erol, A., Suleymanoglu, M., Kuruca, D.S., Savran Oguz, F. (2023). Easy and Rapid Methods for Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells and Human Umbilical Wharton’s Jelly–Derived Mesenchymal Stem Cells. In: Turksen, K. (eds) Stem Cells and Lineage Commitment. Methods in Molecular Biology, vol 2736. Humana, New York, NY. https://doi.org/10.1007/7651_2023_479

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  • DOI: https://doi.org/10.1007/7651_2023_479

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3536-0

  • Online ISBN: 978-1-0716-3537-7

  • eBook Packages: Springer Protocols

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