Abstract
Mesenchymal stem cells show great potential in tissue engineering and cell-based therapies. This protocol demonstrates the use of 3D TableTrix® microcarrier tablets for large-scale manufacturing of human umbilical cord mesenchymal stem cells (hUCMSCs) in a 5-L stirred tank bioreactor. 3D TableTrix® microcarrier tablets readily disperse into tens of thousands of porous microcarriers to simplify cell seeding, and 3D FloTrix® vivaSPIN bioreactor could automate, monitor, and control the entire culture process. 3D TableTrix® microcarriers could also be fully dissolved upon adding dissolution reagent to gently harvest the expanded cells at a high recovery rate. With this protocol, more than 109 cells could be produced in a 5-L bioreactor.
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References
Yin S, Ji C, Wu P, Jin C, Qian H (2019) Human umbilical cord mesenchymal stem cells and exosomes: bioactive ways of tissue injury repair. Am J Transl Res 11(3):1230
Li T, Xia M, Gao Y, Chen Y, Xu Y (2015) Human umbilical cord mesenchymal stem cells: an overview of their potential in cell-based therapy. Expert Opin Biol Ther 15(9):1293–1306. https://doi.org/10.1517/14712598.2015.1051528
Yaghoubi Y, Movassaghpour A, Zamani M, Talebi M, Mehdizadeh A, Yousefi M (2019) Human umbilical cord mesenchymal stem cells derived-exosomes in diseases treatment. Life Sci 233:116733. https://doi.org/10.1016/j.lfs.2019.116733
Lee WY, Wang B (2017) Cartilage repair by mesenchymal stem cells: clinical trial update and perspectives. J Orthop Translat 9:76–88. https://doi.org/10.1016/j.jot.2017.03.005
Lam AT, Lee AP, Jayaraman P, Tan KY, Raghothaman D, Lim HL, Cheng H, Zhou L, Tan AH, Reuveny S, Oh S (2021) Multiomics analyses of cytokines, genes, miRNA, and regulatory networks in human mesenchymal stem cells expanded in stirred microcarrier-spinner cultures. Stem Cell Res 53:102272. https://doi.org/10.1016/j.scr.2021.102272
Mabvuure N, Hindocha S, Khan WS (2012) The role of bioreactors in cartilage tissue engineering. Curr Stem Cell Res Ther 7(4):287–292
Yan X, Zhang K, Yang Y, Deng D, Lyu C, Xu H, Liu W, Du Y (2020) Dispersible and dissolvable porous microcarrier tablets enable efficient large-scale human mesenchymal stem cell expansion. Tissue Eng Part C Methods 26(5):263–275. https://doi.org/10.1089/ten.TEC.2020.0039
Zamani F, Zare Shahneh F, Aghebati-Maleki L, Baradaran B (2013) Induction of CD14 expression and differentiation to monocytes or mature macrophages in Promyelocytic cell lines: new approach. Adv Pharm Bull 3(2):329–332. https://doi.org/10.5681/apb.2013.053
Wang K, Wei G, Liu D (2012) CD19: a biomarker for B cell development, lymphoma diagnosis and therapy. Exp Hematol Oncol 1(1):1–7
Ding DC, Chang YH, Shyu WC, Lin SZ (2015) Human umbilical cord mesenchymal stem cells: a new era for stem cell therapy. Cell Transplant 24(3):339–347. https://doi.org/10.3727/096368915X686841
Kassis I, Zangi L, Rivkin R, Levdansky L, Samuel S, Marx G, Gorodetsky R (2006) Isolation of mesenchymal stem cells from G-CSF-mobilized human peripheral blood using fibrin microbeads. Bone Marrow Transplant 37(10):967–976. https://doi.org/10.1038/sj.bmt.1705358
Acknowledgments
This work is financially supported by Beijing Municipal Science & Technology Commission (Z181100001818005) and R&D program (RC21-01) of Beijing CytoNiche Biotechnology Co. Ltd.
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Xu, H., Cong, Z., Zhang, Y., Liu, W., Yan, X., Du, Y. (2021). Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor. In: Turksen, K. (eds) Bioreactors in Stem Cell Biology. Methods in Molecular Biology, vol 2436. Humana, New York, NY. https://doi.org/10.1007/7651_2021_425
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DOI: https://doi.org/10.1007/7651_2021_425
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2017-5
Online ISBN: 978-1-0716-2018-2
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