Abstract
In the past decades, human induced pluripotent stem cells (iPSCs) have been generated by the ectopic expression of “Yamanaka factors” in multiple somatic cells. However, the procedure to get access to donor cells is hard or invasive in most cases. Hereon, we depict a stepwise method developed in our laboratory for the generation of iPSCs from renal epithelial cells present in urine, which is noninvasive, nonintegrating, and universal. The resulting urinary iPSCs (UiPSCs) exhibit pluripotent characteristics resemble embryonic stem cells (ESCs) and thus urine may be a favorable source for generating iPSCs.
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Acknowledgments
This study was supported by the National Natural Science Foundation of China (82003756, 81770257 and 81970223), the National Key R&D Program of China (2017YFA0103700), the Natural Science Foundation of Jiangsu Province (BK20200880, BK20170002, and BK20201409), the Natural Science Foundation for Colleges and Universities in Jiangsu Province (17KJA310006), Jiangsu Province’s Key Discipline/Laboratory of Medicine (XK201118), National Center for International Research (2017B01012), and Introduction Project of Clinical Medicine Expert Team for Suzhou (SZYJTD201704).
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Yu, M., Han, X., Ye, L., Lei, W., Hu, S. (2021). Generation of Human Induced Pluripotent Stem Cells from Renal Epithelial Cells. In: Turksen, K. (eds) Induced Pluripotent Stem Cells and Human Disease. Methods in Molecular Biology, vol 2549. Humana, New York, NY. https://doi.org/10.1007/7651_2021_419
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DOI: https://doi.org/10.1007/7651_2021_419
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