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A Protocol for Stepwise Differentiation of Induced Pluripotent Stem Cells into Retinal Pigment Epithelium

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Induced Pluripotent Stem Cells and Human Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2549))

Abstract

We have established a stepwise method to differentiate induced pluripotent stem cells (iPSCs) into retinal pigment epithelium (RPE) (iPSC-RPE), which enables efficient isolation and purification of patient-derived iPSC-RPE cells with high quality. Here, we describe in detail the process of differentiating iPSCs into iPSC-RPE.

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References

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Acknowledgments

We thank I. Asaka (CiRA, Kyoto University, Kyoto, Japan) for establishing the iPSC cell lines, N. Harada for technical assistance, K. Iseki and members of the Laboratory for Retinal Regeneration (RIKEN Center for Biosystems Dynamics Research, Kobe, Japan) for useful advice.

This work was partially supported by Grants-in-Aid for Young Scientists [18K16923] (to S.I.).

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Authors and Affiliations

  1. Department of Ophthalmology and Visual Science, Kyoto University Graduate School of Medicine, Kyoto, Japan

    Miho Tagawa, Hanako Ohashi Ikeda, Masayuki Hata, Yumi Inoue, Sachiko Iwai & Akitaka Tsujikawa

Authors
  1. Miho Tagawa
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  2. Hanako Ohashi Ikeda
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  3. Masayuki Hata
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  4. Yumi Inoue
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  5. Sachiko Iwai
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  6. Akitaka Tsujikawa
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Corresponding author

Correspondence to Hanako Ohashi Ikeda .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Tagawa, M., Ikeda, H.O., Hata, M., Inoue, Y., Iwai, S., Tsujikawa, A. (2021). A Protocol for Stepwise Differentiation of Induced Pluripotent Stem Cells into Retinal Pigment Epithelium. In: Turksen, K. (eds) Induced Pluripotent Stem Cells and Human Disease. Methods in Molecular Biology, vol 2549. Humana, New York, NY. https://doi.org/10.1007/7651_2021_418

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  • DOI: https://doi.org/10.1007/7651_2021_418

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2584-2

  • Online ISBN: 978-1-0716-2585-9

  • eBook Packages: Springer Protocols

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