Abstract
Paracellular permeability of the intestinal epithelium is a feature of the intestinal barrier, which plays an important role in the physiology of gut and the whole organism. Intestinal paracellular permeability is controlled by complex processes and is involved in the passage of ions and fluids (called pore pathway) and macromolecules (called leak pathway) through tight junctions, which seal the intercellular space. Impairment of intestinal paracellular permeability is associated with several diseases. The identification of a defect in intestinal paracellular permeability may help to understand the implication of gut barrier as a cause or a consequence in human pathology. Here we describe two complementary methods to evaluate alteration of paracellular permeability in cell culture, using the human intestinal cell line Caco-2 and its clone Caco-2/TC7.
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Acknowledgments
This work was supported by: the Brazilian government’s Science Without Borders Program, the Association François Aupetit (AFA), Institut National de la Santé et de la Recherche Médicale, Sorbonne Université, Ecole Pratique des Hautes Etudes. B.G.P. received a doctoral fellowship (CNPq 207303/2014-2). D.A. received a fellowship from CORDDIM Ile de France.
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Postal, B.G., Aguanno, D., Thenet, S., Carrière, V. (2021). Rapid Evaluation of Intestinal Paracellular Permeability Using the Human Enterocytic-Like Caco-2/TC7 Cell Line. In: Turksen, K. (eds) Permeability Barrier. Methods in Molecular Biology, vol 2367. Humana, New York, NY. https://doi.org/10.1007/7651_2021_366
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DOI: https://doi.org/10.1007/7651_2021_366
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