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Induced Pluripotent Stem (iPS) Cells pp 127–143Cite as

3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells

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Part of the Methods in Molecular Biology book series (MIMB,volume 2454)

Abstract

The generation of cardiomyocytes (CMs) from human pluripotent stem cells (hPSCs) represents a valuable tool for a myriad of in vitro applications, including drug screening, disease modeling and regenerative medicine. However, the success of these applications is dependent on the establishment of reliable, efficient, simple, and cost-effective differentiation methods. In this chapter, we describe an efficient and robust 3D platform for the generation of hPSC-CMs based on the use of a microwell culture system, which can be applied in any laboratory environment. Additionally, we will also describe protocols for the structural and functional characterization of the obtained CMs for further quality control upon differentiation.

Key words

  • Human pluripotent stem cells
  • 3D aggregates
  • Cardiac differentiation
  • Cardiomyocytes
  • Microwells

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  • DOI: 10.1007/7651_2020_336
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Acknowledgments

The authors acknowledge funding from Fundação para a Ciência e a Tecnologia (FCT), Portugal (UIDB/04565/2020) through Programa Operacional Regional de Lisboa 2020 (Project N. 007317) and the project PTDC/EMD-TLM/29728/2017. Mariana A. Branco and João P. Cotovio thank FCT for financial support (PD/BD/128376/2017 and PD/BD135500/2018, respectively).

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Authors and Affiliations

  1. Department of Bioengineering and iBB – Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal

    Mariana A. Branco, Tiago P. Dias, João P. Cotovio, Carlos A. V. Rodrigues, Tiago G. Fernandes, Joaquim M. S. Cabral & Maria Margarida Diogo

Authors
  1. Mariana A. Branco
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  2. Tiago P. Dias
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  3. João P. Cotovio
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  4. Carlos A. V. Rodrigues
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  5. Tiago G. Fernandes
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  6. Joaquim M. S. Cabral
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  7. Maria Margarida Diogo
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Corresponding author

Correspondence to Maria Margarida Diogo .

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Editors and Affiliations

  1. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada

    Dr. Andras Nagy

  2. Ottawa, ON, Canada

    Dr. Kursad Turksen

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Branco, M.A. et al. (2020). 3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells. In: Nagy, A., Turksen, K. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 2454. Humana, New York, NY. https://doi.org/10.1007/7651_2020_336

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  • DOI: https://doi.org/10.1007/7651_2020_336

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2118-9

  • Online ISBN: 978-1-0716-2119-6

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