Abstract
Cell-to-cell communication is an essential process for the efficient function of cells and tissues. Central to this is the purinergic transmission of purines, with ligands such as adenosine triphosphate (ATP). Altered cell-to-cell communication, and in particular changes in the paracrine release of extracellular ATP, plays crucial roles in pathophysiological conditions, such as diabetes. ATP biosensing provides a reliable, real-time measurement of local extracellular ATP concentrations. This allows the detection of altered ATP release, which underlies the progression of inflammation and fibrosis and is a potential therapeutic target. Here we describe in a step-by-step basis how to utilize sensitive microelectrode biosensors to detect low, real-time concentrations of ATP, in vitro.
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Acknowledgments
The authors acknowledge the generous support of Diabetes UK (PES & CEH: 16/0005427, 16/0005544, and 18/0005919), the Royal Society (CEH), and an EFSD/Boehringer Kidney Award (CEH & PES).
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Price, G.W. et al. (2020). Examining Local Cell-to-Cell Signalling in the Kidney Using ATP Biosensing. In: Turksen, K. (eds) Stem Cell Renewal and Cell-Cell Communication. Methods in Molecular Biology, vol 2346. Humana, New York, NY. https://doi.org/10.1007/7651_2020_297
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DOI: https://doi.org/10.1007/7651_2020_297
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