Abstract
Extracellular vesicles (EVs), including exosomes, are nano-sized membrane-bound particles which are released by cells. They have been found in all examined body fluids and can be isolated from conditioned cell culture media. These vesicles have gained increasing attention due to their importance in cellular cross talk, in both health and disease. For example, keratinocyte-derived EVs have been described to modulate melanin production in epidermis. Similar EVs were also shown to have an important role in skin immunology, by stimulating dendritic cells. In this chapter, we will describe how to isolate EVs from keratinocyte cultures and how to perform characterization by Western blot, nanoparticle tracking analysis, and transmission electron microscopy.
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Change history
11 December 2019
The original version of this chapter was inadvertently published with incorrect spelling of surname of the authors. The names should read Sebastian Sjöqvist, Aya Imafuku, Danu Gupta, and Samir EL Andaloussi, and not Sebastian Sjöqvist, Aya Imafuku, Dhanu Ghupta, and Samir E. L. Andaloussi.
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Acknowledgments
This work was supported by the Swedish Society of Medicine. SELA is supported by the Swedish Research Council (VR-Med) and the Swedish foundation of Medical Research (SSF-IRC).
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Sjöqvist, S., Imafuku, A., Gupta, D., EL Andaloussi, S. (2019). Isolation and Characterization of Extracellular Vesicles from Keratinocyte Cultures. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 2109. Humana, New York, NY. https://doi.org/10.1007/7651_2019_264
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DOI: https://doi.org/10.1007/7651_2019_264
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0250-8
Online ISBN: 978-1-0716-0251-5
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