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Scratch Wound Healing Assay

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Epidermal Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2109))


Cell migration is a crucial step for wound healing. Assays able to evaluate cell migration are very useful to evaluate in vitro wound healing. Scratch wound assay creates a gap in confluent monolayer of keratinocytes to mimic a wound. The protocol of scratch wound is based on few steps: cell culture preparation, scratch wound assay, data acquisition, and data analysis.

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  1. Martinotti S, Bucekova M, Majtan J, Ranzato E (2018) Honey: an effective regenerative medicine product in wound management. Curr Med Chem.

    Article  CAS  Google Scholar 

  2. Ranzato E, Martinotti S, Burlando B (2011) Wound healing properties of jojoba liquid wax: an in vitro study. J Ethnopharmacol 134(2):443–449.

    Article  PubMed  Google Scholar 

  3. Bindschadler M, McGrath JL (2007) Sheet migration by wounded monolayers as an emergent property of single-cell dynamics. J Cell Sci 120(Pt 5):876–884.

    Article  CAS  PubMed  Google Scholar 

  4. Grada A, Otero-Vinas M, Prieto-Castrillo F, Obagi Z, Falanga V (2017) Research techniques made simple: analysis of collective cell migration using the wound healing assay. J Invest Dermatol 137(2):e11–e16.

    Article  CAS  PubMed  Google Scholar 

  5. Martinotti S, Calabrese G, Ranzato E (2017) Honeydew honey: biological effects on skin cells. Mol Cell Biochem 435(1–2):185–192.

    Article  CAS  PubMed  Google Scholar 

  6. Pirkmajer B, Leusch G (1977) A bladder-prostate model on which to practice using transurethral resection instruments (author’s transl). Urologe A 16(6):336–338

    CAS  PubMed  Google Scholar 

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Correspondence to Elia Ranzato .

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© 2019 Springer Science+Business Media New York

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Martinotti, S., Ranzato, E. (2019). Scratch Wound Healing Assay. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 2109. Humana, New York, NY.

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0250-8

  • Online ISBN: 978-1-0716-0251-5

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