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Single Cell Densitometry and Weightlessness Culture of Mesenchymal Stem Cells Using Magnetic Levitation

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 2125)

Abstract

Magnetic levitation methodology enables density-based separation of microparticles/cells and sustains cell culture in different media. Levitation process can be accomplished via negative magnetophoresis (diamagnetophoresis), where the applied magnetic force compensates gravitational acceleration and the density of the diamagnetic object (e.g., cell) determines its levitation height. Here we describe a portable, sensitive, and cost-effective technology that uses the principles of magnetic levitation to measure single cell density and cell culture under desired conditions.

Keywords

Magnetic levitation Single cell density Simulated microgravity Stem cells Real-time monitoring 
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Notes

Acknowledgements

This work was financially supported by the Scientific and Technological Research Council of Turkey (116M298, HCT and 215S862, EO).

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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  1. 1.Department of BioengineeringIzmir Institute of TechnologyUrla, IzmirTurkey

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