Abstract
Ever since the discovery of stem cells, their isolation from tissues and expansion in culture has been extensively studied due to its potential for therapeutic application. The magnetic-assisted cell sorting (MACS) method is the most widely used technique for the sorting of cells based on their cell surface markers. Though effective, the major drawbacks are high cost and the requirement for the frequent replacement of the columns. In the column-free method, the cells are sorted using the same principle of immune-magnetic isolation but does not require magnetic columns, making it cost-effective. The isolation of c-kit+ stem cells from atrial explants using column-free magnet is found to be efficient and yields homogenous population of stem cells. This method saves time and labor and is economical when working with large sample sizes.
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References
He J-Q, Vu DM, Hunt G et al (2011) Human cardiac stem cells isolated from atrial appendages stably express c-kit. PLoS One 6:e27719
Saheera S, Nair RR (2017) Accelerated decline in cardiac stem cell efficiency in spontaneously hypertensive rat compared to normotensive Wistar rat. PLoS One 12:e0189129
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Saheera, S., Nair, R.R. (2019). Column-Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants. In: Turksen, K. (eds) Stem Cells and Aging . Methods in Molecular Biology, vol 2045. Humana, New York, NY. https://doi.org/10.1007/7651_2019_207
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DOI: https://doi.org/10.1007/7651_2019_207
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9712-1
Online ISBN: 978-1-4939-9713-8
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