Abstract
Endothelial cell culture under flow, to mimic physiological conditions within blood vessels, has gained particular attention for the formation of a homogeneous endothelium in vitro. Here, we report on the design of a setup for simultaneous culture of up to nine electrospun membranes or thin polymer films in custom-made holders under flow on an orbital shaker. The versatile design of the device allows for the use of electrospun membranes/polymer films of choice and subsequent analysis with commonly used methods such as immunofluorescence or scanning electron microscopy.
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Acknowledgments
We would like to thank Jörg Gschwend, Empa, St. Gallen for material manufacture and his assistance for device design. We are also grateful to Marielle Hintereder, Empa, St. Gallen, for assistance during cell culture. Furthermore, we would like to acknowledge the input from Andreia Fernandes and Seyedvahid Hosseini, Laboratory for Applied Mechanobiology, ETH Zürich for setting up cell culture on an orbital shaker. This work is part of the Zurich Heart project under the umbrella of University Medicine Zurich/Hochschulmedizin Zürich.
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Mertgen, AS., Rottmar, M., Weidenbacher, L., Guex, A.G. (2018). Design of a Versatile Sample Holder for Facile Culture of Cells on Electrospun Membranes or Thin Polymer Films Under Flow Conditions. In: Turksen, K. (eds) Stem Cell Nanotechnology. Methods in Molecular Biology, vol 2125. Humana, New York, NY. https://doi.org/10.1007/7651_2018_192
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DOI: https://doi.org/10.1007/7651_2018_192
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0359-8
Online ISBN: 978-1-0716-0360-4
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