Identification and Characterization of Stem Cells in Oral Cancer
Cancer stem cells (CSCs) are a subpopulation of cells within a heterogeneous tumor that have enhanced biologic properties such as increased capacity for self-renewal, increased tumorigenicity, enhanced differentiation capacity, and resistance to chemo- and radiotherapies. This unit describes protocols to isolate and characterize potential cancer stem cells from a solid tumor (oral cancer). This involves creating a single-cell suspension from tumor tissue, tagging the cell subpopulation of interest, and sorting cells into different populations. Finally, the sorted subpopulations can be evaluated for their ability to meet the functional requirements of a CSC, which primarily include increased tumorigenicity in an in vivo xenograft assay. Mastering the protocols in this unit will allow the researcher to study populations of cells that may have properties of CSCs.
KeywordsAldehyde dehydrogenase activity Cancer stem cells CD44 Orosphere Side population Xenograft
Research support was partly provided by Board of Research in Nuclear Sciences (BRNS) [Number: 37(1)/14/38/2016-BRNS/37276], Department of Atomic Energy (DAE), and Science and Engineering Research Board (SERB) [Number: EMR/2016/001246]. Research infrastructure was partly provided by Fund for Improvement of S&T Infrastructure in Universities and Higher Educational Institutions (FIST) [Number: SR/FST/LSI-025/2014], Department of Science and Technology, Government of India.
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