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Skin Stem Cells pp 211–219Cite as

Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1879))

Abstract

Various natural and synthetic biomaterials have been applied as skin substitutes for regenerating damaged skin. Here, we describe a straightforward method for fabrication of a tissue-engineered skin substitute by seeding human fetal fibroblasts on acellular human amniotic membrane (HAM). Fetal fibroblasts are achieved from the skin of normal and non-macerated fetus of 11–14 weeks old after spontaneous pregnancy termination. Acellular HAM is obtained by separation of the outer membrane of the chorion and removing its epithelial cells.

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Acknowledgments

The protocols described here were developed under the support of a Royan institute grant.

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Authors and Affiliations

  1. Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

    Ehsan Taghiabadi, Nasser Aghdami & Amir Bajouri

  2. Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Ehsan Taghiabadi & Bahareh Beiki

Authors
  1. Ehsan Taghiabadi
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  2. Bahareh Beiki
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  3. Nasser Aghdami
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  4. Amir Bajouri
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Corresponding author

Correspondence to Amir Bajouri .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Taghiabadi, E., Beiki, B., Aghdami, N., Bajouri, A. (2018). Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_135

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  • DOI: https://doi.org/10.1007/7651_2018_135

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8869-3

  • Online ISBN: 978-1-4939-8870-9

  • eBook Packages: Springer Protocols

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