pp 1-7 | Cite as

Determining Dissolved and Biogenic Silica

  • Jaana Koistinen
  • Mervi Sjöblom
  • Kristian Spilling
Part of the Methods in Molecular Biology book series


Most algae do not use silicon in any form with one notable exception, diatoms. Silicon is a major constituent of diatoms. Diatoms are characterized by high growth rates and are often one of the key groups in forming algal blooms in natural waters, and as such it is an interesting group for cultivation. In this chapter we present methods for determining dissolved silica (DSi) and biogenic silica (BSi), oxide forms of silicon, based on colorimetric methods. BSi is determined after filtration and alkaline digestion.


Biogenic silica Diatoms Dissolved silica Silicon 


  1. 1.
    Hildebrand M, Davis AK, Smith SR, Traller JC, Abbriano R (2012) The place of diatoms in the biofuels industry. Biofuels 3(2):221–240CrossRefGoogle Scholar
  2. 2.
    Bozarth A, Maier UG, Zauner S (2009) Diatoms in biotechnology: modern tools and applications. Appl Microbiol Biotechnol 82(2):195–201CrossRefPubMedGoogle Scholar
  3. 3.
    Armbrust EV (2009) The life of diatoms in the world’s oceans. Nature 459:185–192ADSCrossRefPubMedGoogle Scholar
  4. 4.
    Martin-Jézéquel V, Hildebrand M, Brzezinski MA (2000) Silicon metabolism in diatoms: implications for growth. J Phycol 36:821–840CrossRefGoogle Scholar
  5. 5.
    Round FE, Crawford RM, Mann DG (2007) Diatoms: biology and morphology of the genera. Cambridge University Press, CambridgeGoogle Scholar
  6. 6.
    Grasshoff K, Ehrhardt M, Kremling K (1999) Methods of seawater analysis. Wiley-VCH Verlag Gmbh, WeinheimCrossRefGoogle Scholar
  7. 7.
    Krausse GL, Schelske CL, Davis CO (1983) Comparisons of three wet-alkaline methods of digestion of biogenic silica in water. Freshwater Biol 13:73–81CrossRefGoogle Scholar
  8. 8.
    Koroleff F (1979) Methods for the chemical analysis for seawater. Meri 7:1–60. (In Finnish)Google Scholar
  9. 9.
    Haeckel EHPA (1904) Kunstformen der Natur. Bibliographisches Institut, LeipzigCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Jaana Koistinen
    • 1
  • Mervi Sjöblom
    • 1
  • Kristian Spilling
    • 2
  1. 1.Tvärminne Zoological StationUniversity of HelsinkiHankoFinland
  2. 2.Finnish Environment InstituteHelsinkiFinland

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