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Basic Methods for Isolating and Culturing Microalgae

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Biofuels from Algae

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1980))

Abstract

In this chapter, I go through the very basic methods for isolating and setting up a microalgae culture. Isolation can be done from local algal strains with a micropipette and microscope. Determining algal growth is important and can be done by counting cells under a microscope or by determining the change in dry weight (DW).

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References

  1. Sheehan J, Dunahay T, Benemann J, Roessler P (1998) A look back at the US Department of Energy’s Aquatic Species Program: biodiesel from algae. National Renewable Energy Laboratory Report NREL/TP-580-24190

    Google Scholar 

  2. Williams PJ lB, Laurens LML (2010) Microalgae as biodiesel & biomass feedstocks: review & analysis of the biochemistry, energetics & economics. Energy Environ Sci 3:554–590

    Article  CAS  Google Scholar 

  3. Nalley JO, Stockenreiter M, Litchman E (2014) Community ecology of algal biofuels: complementarity and trait-based approaches. Ind Biotechnol 10:191–201

    Article  Google Scholar 

  4. Harrison PJ, Berges JA (2005) Marine culture media. In: Andersen RA (ed) Algal culturing techniques. Academic Press, Cambridge

    Google Scholar 

  5. Burlew JS (ed) (1953) Algal culture, from laboratory to pilot plant. Carnegie Institution of Washington Publication, Washington, DC

    Google Scholar 

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Correspondence to Kristian Spilling .

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Spilling, K. (2017). Basic Methods for Isolating and Culturing Microalgae. In: Spilling, K. (eds) Biofuels from Algae. Methods in Molecular Biology, vol 1980. Humana, New York, NY. https://doi.org/10.1007/7651_2017_99

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  • DOI: https://doi.org/10.1007/7651_2017_99

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9415-1

  • Online ISBN: 978-1-4939-9416-8

  • eBook Packages: Springer Protocols

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