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In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering

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Decellularized Scaffolds and Organogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1577))

Abstract

Whole-organ decellularization is recently gaining interest in the transplantation field as strategy to obtain acellular scaffold only composed by extracellular matrix. These structures, that still remain organ-specific in terms of biological cues and tridimensional morphology could be then recellularized with patient's autologous cells. The final result should be a nwe transplantable autologous organ that should by-pass, at the same time, the problem of organ shortage and secondly the consequences related to the immunosuppression need. Herein we describe the protocol to manufacture a whole-organ transplantable rat kidney scaffold by a dual-detergent (Triton X-100 and SDS) arterial peristaltic perfusion. Final results show whole-renal acellular scaffold with contextual preservation of tridimensional architecture and biological properties deriving from the extracellular matrix composition.

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References

  1. World Health Organization (2013) World Health Statistic. World Health Organization, Geneve, Switzerland

    Google Scholar 

  2. Beuscart JB, Pagniez D, Boulanger E, Duhamel A (2015) Registration on the renal transplantation waiting list and mortality on dialysis: an analysis of the French REIN registry using a multi-state model. J Epidemiol 25:133–141

    Article  Google Scholar 

  3. Liyanage T, Ninomiya T, Jha V, Neal B, Patrice HM, Okpechi I, Zhao MH, Lv J, Garg AX, Knight J, Rodgers A, Gallagher M, Kotwal S, Cass A, Perkovic V (2015) Worldwide access to treatment for end-stage kidney disease: a systematic review. Lancet 385:1975–1982

    Article  Google Scholar 

  4. Crapo PM, Gilbert TW, Badylak SF (2011) An overview of tissue and whole organ decellularization processes. Biomaterials 32:3233–3243

    Article  CAS  Google Scholar 

  5. Page-McCaw A, Ewald AJ, Werb Z (2007) Matrix metalloproteinases and the regulation of tissue remodelling. Nat Rev Mol Cell Biol 8:221–233

    Article  CAS  Google Scholar 

  6. Gilbert TW, Wognum S, Joyce EM, Freytes DO, Sacks MS, Badylak SF (2008) Collagen fiber alignment and biaxial mechanical behavior of porcine urinary bladder derived extracellular matrix. Biomaterials 29:4775–4782

    Article  CAS  Google Scholar 

  7. Xu CC, Chan RW, Tirunagari N (2007) A biodegradable, acellular xenogeneic scaffold for regeneration of the vocal fold lamina propria. Tissue Eng 13:551–566

    Article  CAS  Google Scholar 

  8. Dong X, Wei X, Yi W, Gu C, Kang X, Liu Y, Li Q, Yi D (2009) RGD-modified acellular bovine pericardium in a bioprosthetic scaffold for tissue engineering. J Mater Sci Mater Med 20:2327–2336

    Article  CAS  Google Scholar 

  9. Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB, Gavrilov K, Yi T, Zhuang ZW, Breuer C, Herzog E, Niklason LE (2010) Tissue-engineered lungs for in vivo implantation. Science 329:538–541

    Article  CAS  Google Scholar 

  10. Lehr EJ, Rayat GR, Chiu B, Churchill T, McGann LE, Coe JY, Ross DB (2010) Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches. J Thorac Cardiovasc Surg 141:1056–1062

    Article  Google Scholar 

  11. Dahl SL, Koh J, Prabhakar V, Niklason LE (2003) Decellularized native and engineered arterial scaffolds for transplantation. Cell Transplant 12:659–666

    Article  Google Scholar 

  12. Bolland F, Korossis S, Wilshaw SP, Ingham E, Fisher J, Kearney JN, Southgate J (2007) Development and characterization of a full-thickness acellular porcine bladder matrix for tissue engineering. Biomaterials 28:1061–1070

    Article  CAS  Google Scholar 

  13. Henderson PW, Nagineni VV, Harper A, Bavinck N, Sohn AM, Krijgh DD, Jimenez N, Weinstein AL, Spector JA (2010) Development of an acellular bioengineered matrix with a dominant vascular pedicle. J Surg Res 164:1–5

    Article  CAS  Google Scholar 

  14. Shupe T, Williams M, Brown A, Willenberg B, Petersen BE (2010) Method for the decellularization of intact rat liver. Organogenesis 6:134–136

    Article  Google Scholar 

  15. Akhyari P, Aubin H, Gwanmesia P, Barth M, Hoffmann S, Huelsmann J, Preuss K, Lichtenberg A (2011) The quest for an optimized protocol for whole-heart decellularization: a comparison of three popular and a novel decellularization technique and their diverse effects on crucial extracellular matrix qualities. Tissue Eng Part C Methods 17:915–926

    Article  CAS  Google Scholar 

  16. Ott HC, Matthiesen TS, Goh SK, Black LD, Kren SM, Netoff TI, Taylor DA (2008) Perfusion-decellularized matrix: using nature’s platform to engineer a bioartificial heart. Nat Med 14:213–221

    Article  CAS  Google Scholar 

  17. Peloso A, Ferrario J, Maiga B, Benzoni I, Bianco C, Citro A, Currao M, Malara A, Gaspari A, Balduini A, Abelli M, Piemonti L, Dionigi P, Orlando G, Maestri M (2015) Creation and implantation of acellular rat renal ECM based scaffolds. Organogenesis 11:58–74

    Article  Google Scholar 

  18. Chen MK, Badylak SF (2001) Small bowel tissue engineering using small intestinal submucosa as a scaffold. J Surg Res 99:352–358

    Article  CAS  Google Scholar 

  19. Goh SK, Bertera S, Olsen P, Candiello JE, Halfter W, Uechi G, Balasubramani M, Johnson SA, Sicari BM, Kollar E, Badylak SF, Banerjee I (2013) Perfusion-decellularized pancreas as a natural 3D scaffold for pancreatic tissue and whole organ engineering. Biomaterials 34:6760–6772

    Article  CAS  Google Scholar 

  20. Peloso A, Urbani L, Cravedi P, Katari R, Maghsoudlou P, Fallas ME, Sordi V, Citro A, Purroy C, Niu G, McQuilling JP, Sittadjody S, Farney AC, Iskandar SS, Zambon JP, Rogers J, Stratta RJ, Opara EC, Piemonti L, Furdui CM, Soker S, De Coppi P, Orlando G (2016) The human pancreas as a source of protolerogenic extracellular matrix scaffold for a new-generation bioartificial endocrine pancreas. Ann Surg 264:169–179

    Article  Google Scholar 

  21. Peloso A, Petrosyan A, Da Sacco S, Booth C, Zambon JP, O'Brien T, Aardema C, Robertson J, De Filippo RE, Soker S, Stratta RJ, Perin L, Orlando G (2015) Renal extracellular matrix scaffolds from discarded kidneys maintain glomerular morphometry and vascular resilience and retains critical growth factors. Transplantation 99:1807–1816

    Article  CAS  Google Scholar 

  22. Mazza G, Rombouts K, Rennie Hall A, Urbani L, Vinh Luong T, Al-Akkad W, Longato L, Brown D, Maghsoudlou P, Dhillon AP, Fuller B, Davidson B, Moore K, Dhar D, De Coppi P, Malago M, Pinzani M (2015) Decellularized human liver as a natural 3D-scaffold for liver bioengineering and transplantation. Sci Rep 5:13079

    Article  CAS  Google Scholar 

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Acknowledgments

AP was supported by an Investigator Fellowship grant from Collegio Ghislieri, Pavia, Italy.

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Authors and Affiliations

  1. Department of Clinical, Surgical, Diagnostic and Paediatric Sciences, University of Pavia, Pavia, Italy

    Andrea Peloso & Lorenzo Cobianchi

  2. Department of General Surgery, IRCCS Policlinico San Matteo, Pavia, Italy

    Andrea Peloso, Francesco Calabrese, Tommaso Dominioni, Marcello Maestri & Lorenzo Cobianchi

  3. Divisions of Abdominal and Transplantation Surgery, Hepato-Pancreato-Biliary Centre, Department of Surgery, Faculty of Medicine, University Hospital of Geneva, University of Geneva, Geneva, Switzerland

    Andrea Peloso & Graziano Oldani

  4. San Raffaele Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, Milan, Italy

    Antonio Citro

  5. IRCCS Policlinico San Matteo, Unit of Nephrology, Dialysis and Transplantation, Pavia, Italy

    Valeria Corradetti

  6. University of Pavia, Pavia, Italy

    Szandra Brambilla

Authors
  1. Andrea Peloso
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  2. Antonio Citro
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  3. Valeria Corradetti
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  4. Szandra Brambilla
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  5. Graziano Oldani
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  6. Francesco Calabrese
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  7. Tommaso Dominioni
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  8. Marcello Maestri
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  9. Lorenzo Cobianchi
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Corresponding author

Correspondence to Andrea Peloso .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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Peloso, A. et al. (2017). In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_96

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  • DOI: https://doi.org/10.1007/7651_2017_96

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7655-3

  • Online ISBN: 978-1-4939-7656-0

  • eBook Packages: Springer Protocols

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