Abstract
Because of our access to human genome data and ever-improving genome sequencing and proteome analysis methods, we are much better in terms of our understanding of biological processes. In addition to genomics, proteomics, and other “omics” methods, availability of more sophisticated molecular assaying methods has augmented our knowledge about immune processes toward auto- and allogeneic targets. High-density protein arrays are developed to analyze protein-small molecule interactions, enzyme-substrate profiling, protein-protein interaction, and immune monitoring by assessing antibodies in the serum.
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References
Sigdel TK, Sarwal MM (2011) Recent advances in biomarker discovery in solid organ transplant by proteomics. Expert Rev Proteomics 8(6):705–715
Mattoon D et al (2005) Biomarker discovery using protein microarray technology platforms: antibody-antigen complex profiling. Expert Rev Proteomics 2(6):879–889
Li L et al (2009) Identifying compartment-specific non-HLA targets after renal transplantation by integrating transcriptome and “antibodyome” measures. Proc Natl Acad Sci U S A 106(11):4148–4153
Li L et al (2010) Compartmental localization and clinical relevance of MICA antibodies after renal transplantation. Transplantation 89(3):312–319
Li L et al (2010) Differential immunogenicity and clinical relevance of kidney compartment specific antigens after renal transplantation. J Proteome Res 9(12):6715–6721
Sigdel TK et al (2012) Non-HLA antibodies to immunogenic epitopes predict the evolution of chronic renal allograft injury. J Am Soc Nephrol 23(4):750–763
Sigdel TK et al (2011) Profiling of autoantibodies in IgA nephropathy, an integrative antibiomics approach. Clin J Am Soc Nephrol 6(12):2775–2784
Sutherland SM et al (2009) Protein microarrays identify antibodies to protein kinase Czeta that are associated with a greater risk of allograft loss in pediatric renal transplant recipients. Kidney Int 76(12):1277–1283
Tusher VG, Tibshirani R, Chu G (2001) Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci U S A 98(9):5116–5121
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Sigdel, T.K., Sarwal, M.M. (2017). Discovery of Immune Reactive Human Proteins by High-Density Protein Arrays and Customized Validation of Potential Biomarkers by ELISA. In: Sarwal, M., Sigdel, T. (eds) Tissue Proteomics. Methods in Molecular Biology, vol 1788. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_92
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DOI: https://doi.org/10.1007/7651_2017_92
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7852-6
Online ISBN: 978-1-4939-7854-0
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