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Tissue Proteomics pp 23–41Cite as

HLA Class I and Class II-Induced Intracellular Signaling and Molecular Associations in Primary Human Endothelial Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1788))

Abstract

The signaling capacity of HLA molecules in vascular cells has been well established. Intracellular signaling and association with the coreceptor integrin β4 has been well-studied for HLA class I. However, little is known regarding HLA class II intracellular signaling in human endothelial cells. Investigation of HLA class II has been challenging due to the loss of HLA class II expression in cultured primary cells. Herein, we describe methods for inducing expression of endogenous alleles and loci of HLA class II molecules, as well as for studying intracellular signaling. This includes siRNA knockdown of proteins and coimmunoprecipitation of putative coreceptors for HLA in primary human aortic endothelial cells.

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Authors and Affiliations

  1. Immunogenetics Center, UCLA Pathology and Laboratory Medicine, Los Angeles, CA, 90095, USA

    Nicole Valenzuela, Nwe Nwe Soe, Fang Li & Yi-Ping Jin

  2. Cedars Sinai, Los Angeles, CA, USA

    Xiaohai Zhang

  3. UCLA Pathology and Laboratory Medicine, 10833 Le Conte Ave., CHS 14-127, Los Angeles, CA, 90095-1732, USA

    Elaine F. Reed

Authors
  1. Nicole Valenzuela
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  2. Nwe Nwe Soe
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  3. Fang Li
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  4. Xiaohai Zhang
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  5. Yi-Ping Jin
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  6. Elaine F. Reed
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Corresponding author

Correspondence to Elaine F. Reed .

Editor information

Editors and Affiliations

  1. Professor, Department of Surgery Medicine and Pediatrics Director, Kidney-Pancreas Transplant Program, University of California San Francisco, San Francisco, California, USA

    Minnie M. Sarwal

  2. Asst. Professor, Department of Surgery, University of California San Francisco, San Francisco, California, USA

    Tara K. Sigdel

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Valenzuela, N., Soe, N.N., Li, F., Zhang, X., Jin, YP., Reed, E.F. (2017). HLA Class I and Class II-Induced Intracellular Signaling and Molecular Associations in Primary Human Endothelial Cells. In: Sarwal, M., Sigdel, T. (eds) Tissue Proteomics. Methods in Molecular Biology, vol 1788. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_79

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  • DOI: https://doi.org/10.1007/7651_2017_79

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7852-6

  • Online ISBN: 978-1-4939-7854-0

  • eBook Packages: Springer Protocols

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