There is a rapidly growing interest in generation of 3D organotypic microtissues with human physiologically relevant structure, function, and cell population in a wide range of applications including drug screening, in vitro physiological/pathological models, and regenerative medicine. Here, we provide a detailed procedure to generate structurally defined 3D organotypic microtissues from cells or cell spheroids using acoustic waves as a biocompatible and scaffold-free tissue engineering tool.
3D organotypic microtissues Acoustic assembly Acoustic waves Cardiac scaffold Cardiomyocyte Cell assembly Cell spheroids In vitro tissue model Regenerative medicine Spheroid assembly Tissue engineering
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U.D. acknowledges that this material is based in part upon work supported by the NSF CAREER Award Number 1150733. S.G. would like to thank the Turkish Scientific Research and Technology Council (TÜBİTAK) award number 115C125, the Turkish Academy of Sciences GEBIP Award, and the Academy of Sciences BAGEP Award. P.C. and Y. Z. would like to acknowledge Wuhan University Program 2042017kf0230. V.S. acknowledges NIH Pathway to Independence Award 1K99HL127295-01A1.
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