pp 1-12 | Cite as

Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly

Protocol
Part of the Methods in Molecular Biology book series

Abstract

There is a rapidly growing interest in generation of 3D organotypic microtissues with human physiologically relevant structure, function, and cell population in a wide range of applications including drug screening, in vitro physiological/pathological models, and regenerative medicine. Here, we provide a detailed procedure to generate structurally defined 3D organotypic microtissues from cells or cell spheroids using acoustic waves as a biocompatible and scaffold-free tissue engineering tool.

Keywords

3D organotypic microtissues Acoustic assembly Acoustic waves Cardiac scaffold Cardiomyocyte Cell assembly Cell spheroids In vitro tissue model Regenerative medicine Spheroid assembly Tissue engineering 

Notes

Acknowledgments

U.D. acknowledges that this material is based in part upon work supported by the NSF CAREER Award Number 1150733. S.G. would like to thank the Turkish Scientific Research and Technology Council (TÜBİTAK) award number 115C125, the Turkish Academy of Sciences GEBIP Award, and the Academy of Sciences BAGEP Award. P.C. and Y. Z. would like to acknowledge Wuhan University Program 2042017kf0230. V.S. acknowledges NIH Pathway to Independence Award 1K99HL127295-01A1.

References

  1. 1.
    Guven S et al (2015) Multiscale assembly for tissue engineering and regenerative medicine. Trends Biotechnol 33(5):269–279CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Esch EW, Bahinski A, Huh D (2015) Organs-on-chips at the frontiers of drug discovery. Nat Rev Drug Discov 14(4):248–260CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Asghar W et al (2013) In vitro three-dimensional cancer culture models. In: Bae YH, Mrsny RJ, Park K (eds) Cancer targeted drug delivery: an elusive dream. Springer, New York, NY, pp 635–665Google Scholar
  4. 4.
    Arslan-Yildiz A et al (2016) Towards artificial tissue models: past, present, and future of 3D bioprinting. Biofabrication 8(1):014103ADSCrossRefPubMedGoogle Scholar
  5. 5.
    Serpooshan V, Mahmoudi M, Hu DA, Hu JB, Wu SM (2017) Bioengineering cardiac constructs using 3D printing. J 3D Printing Med 1(2):123–139CrossRefGoogle Scholar
  6. 6.
    Chen P, Guven S, Usta OB, Yarmush ML, Demirci U (2015) Biotunable acoustic node assembly of organoids. Adv Healthc Mater 4(13):1937–1943CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Chen P et al (2014) Microscale assembly directed by liquid-based template. Adv Mater 26(34):5936–5941CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Serpooshan V et al (2017) Bioacoustic-enabled patterning of human iPSC-derived cardiomyocytes into 3D cardiac tissue. Biomaterials 131:47–57CrossRefPubMedGoogle Scholar
  9. 9.
    Lee S et al (2017) Contractile force generation by 3D hiPSC-derived cardiac tissues is enhanced by rapid establishment of cellular interconnection in matrix with muscle-mimicking stiffness. Biomaterials 131:111–120ADSCrossRefPubMedGoogle Scholar
  10. 10.
    Burridge PW et al (2014) Chemically defined generation of human cardiomyocytes. Nat Methods 11(8):855–860CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Chase LCJDP (2015) Epigenetic regulation of phosphodiesterases 2A and 3A underlies compromised b-adrenergic signaling in an iPSC model of dilated cardiomyopathy. Front Physiol 7Google Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Biomedical Engineering, School of Basic Medical SciencesWuhan UniversityWuhanChina
  2. 2.Institute of Model Animals of Wuhan UniversityWuhanChina
  3. 3.Stanford Cardiovascular InstituteStanford University School of MedicineStanfordUSA
  4. 4.Bio-Acoustic MEMS in Medicine (BAMM) Lab, Department of Radiology, Canary Center for Early Cancer DetectionStanford University School of MedicineStanfordUSA
  5. 5.Izmir International Biomedicine and Genome InstituteDokuz Eylul UniversityIzmirTurkey
  6. 6.Department of Medical Biology, Faculty of MedicineDokuz Eylul UniversityIzmirTurkey

Personalised recommendations