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Tracheal Cartilage Isolation and Decellularization

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Book cover Decellularized Scaffolds and Organogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1577))

Abstract

Tissue decellularization allows isolation of tissue extracellular matrix components, enabling bioengineering of native tissue microenvironments with minimal antigenic components. Here we describe a method to harvest decellularized cartilage tissue from donor trachea using a series of chemical and enzymatic washes and incorporating the extracellular matrix in gelatin methacrylate hydrogels. This decellularized cartilage tissue is easily incorporated into a variety of hydrogels to create a cartilage tissue scaffold.

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References

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Acknowledgments

We would like to thank Daniel Sorby for his help procuring animal tissue and processing. We would also like to thank the Hormel company (Austin, MN) for their generosity, donating porcine trachea for research.

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Authors and Affiliations

  1. Department of Pediatrics, Blood and Marrow Transplant Program, University of Minnesota, Mayo Mail Code 366, 420 Delaware Street SE, Minneapolis, MN, 55455, USA

    Zachary Galliger & Angela Panoskaltsis-Mortari

Authors
  1. Zachary Galliger
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  2. Angela Panoskaltsis-Mortari
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Corresponding author

Correspondence to Angela Panoskaltsis-Mortari .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2017 Springer Science+Business Media New York

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Galliger, Z., Panoskaltsis-Mortari, A. (2017). Tracheal Cartilage Isolation and Decellularization. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_52

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  • DOI: https://doi.org/10.1007/7651_2017_52

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7655-3

  • Online ISBN: 978-1-4939-7656-0

  • eBook Packages: Springer Protocols

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