Protocol

Part of the series Methods in Molecular Biology pp 1-7

Date:

Mouse Skeletal Muscle Decellularization

  • Martina PiccoliAffiliated withFondazione Istituto di Ricerca Pediatrica Città della Speranza
  • , Caterina TrevisanAffiliated withFondazione Istituto di Ricerca Pediatrica Città della SperanzaDepartment of Women and Children Health, University of Padova
  • , Edoardo MaghinAffiliated withFondazione Istituto di Ricerca Pediatrica Città della Speranza
  • , Chiara FranzinAffiliated withFondazione Istituto di Ricerca Pediatrica Città della Speranza
  • , Michela PozzobonAffiliated withFondazione Istituto di Ricerca Pediatrica Città della SperanzaDepartment of Women and Children Health, University of PadovaStem Cells and Regenerative Medicine Lab, Istituto di Ricerca Pediatrica Città della Speranza Email author 

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Abstract

Natural acellular matrices obtained from decellularization procedures are biocompatible and non-immunogenic materials considered promising tools for regenerative medicine purposes. Before in vivo implantation, these matrices must be efficiently decellularized, removing all the cellular components to avoid any immunogenic reaction. At the same time, it is important to maintain the original three-dimensional structure of the specific tissue. Here we describe a method: (1) to decellularize mouse quadriceps using a detergent-enzymatic treatment (DET) and (2) to assess decellularization efficiency and scaffold properties.

Keywords:

Decellularization Extracellular matrix Mouse skeletal muscle Volumetric muscle loss Tissue engineering