pp 1-8 | Cite as

A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration

Protocol
Part of the Methods in Molecular Biology book series

Abstract

Traditional ex vivo expansion of adult stem cells yields an insufficient quantity of less potent cells. Here we describe the fabrication of decellularized matrix deposited by synovium-derived stem cells (SDSCs). This matrix could serve as a three-dimensional expansion system to rejuvenate cells for proliferation and tissue-specific differentiation potential, which could benefit cartilage regeneration. The decellularized stem cell matrix (DSCM) might be a powerful system for tissue engineering and regeneration.

Keywords

Decellularized stem cell matrix Cell expansion Synovium-derived stem cells Chondrogenesis Cartilage tissue engineering Cartilage regeneration 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, Division of Exercise Physiology, and Mechanical and Aerospace EngineeringWest Virginia UniversityMorgantownUSA
  2. 2.Stem Cell and Tissue Engineering Laboratory, Department of OrthopaedicsWest Virginia UniversityMorgantownUSA

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