pp 1-8 | Cite as

Monitoring of Atg5-Independent Mitophagy

  • Satoko Arakawa
  • Shinya Honda
  • Satoru Torii
  • Masatsune Tsujioka
  • Shigeomi Shimizu
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Mitophagy is a mitochondrial quality control mechanism where damaged and surplus mitochondria are removed by autophagy. There are at least two different mammalian autophagy pathways: the Atg5-dependent conventional pathway and an Atg5-independent alternative pathway; the latter is involved in the erythrocyte mitophagy. In this chapter we describe the various experimental approaches to assess Atg5-indepedndent mitophagy in mammalian cells.

Keywords:

Mitophagy Atg5-independent autophagy Erythrocyte maturation Parkin Selective autophagy 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Satoko Arakawa
    • 1
  • Shinya Honda
    • 1
  • Satoru Torii
    • 1
  • Masatsune Tsujioka
    • 1
  • Shigeomi Shimizu
    • 1
  1. 1.Department of Pathological Cell Biology, Medical Research InstituteTokyo Medical and Dental UniversityTokyoJapan

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