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Organoids pp 205–213Cite as

Oncogenic Transformation of Human-Derived Gastric Organoids

Part of the Methods in Molecular Biology book series (MIMB,volume 1576)


The culture of organoids has represented a significant advancement in the gastrointestinal research field. Previous research studies have described the oncogenic transformation of human intestinal and mouse gastric organoids. Here we detail the protocol for the oncogenic transformation and orthotopic transplantation of human-derived gastric organoids.


  • Human fundic gastric organoids
  • CRISPR/Cas-9
  • Nucleofection
  • Organoid orthotopic transplantation

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This work was supported by NIH 1R01DK083402 grant and College of Medicine Bridge Funding Program (Zavros), NIH 5T32GM105526, Albert J. Ryan Fellowship, and Dean’s Fellowship (Bertaux-Skeirik).

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Correspondence to Yana Zavros Ph.D. .

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Bertaux-Skeirik, N., Centeno, J., Gao, J., Gabre, J., Zavros, Y. (2016). Oncogenic Transformation of Human-Derived Gastric Organoids. In: Turksen, K. (eds) Organoids. Methods in Molecular Biology, vol 1576. Humana, New York, NY.

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7616-4

  • Online ISBN: 978-1-4939-7617-1

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