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Oncogenic Transformation of Human-Derived Gastric Organoids

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Organoids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1576))

Abstract

The culture of organoids has represented a significant advancement in the gastrointestinal research field. Previous research studies have described the oncogenic transformation of human intestinal and mouse gastric organoids. Here we detail the protocol for the oncogenic transformation and orthotopic transplantation of human-derived gastric organoids.

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Acknowledgements

This work was supported by NIH 1R01DK083402 grant and College of Medicine Bridge Funding Program (Zavros), NIH 5T32GM105526, Albert J. Ryan Fellowship, and Dean’s Fellowship (Bertaux-Skeirik).

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Authors and Affiliations

  1. Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, OH, USA

    Nina Bertaux-Skeirik

  2. Biology Department, University of Puerto Rico-Río Piedras, San Juan, Puerto Rico

    Jomaris Centeno

  3. Department of Pediatrics, W.F. Maternal & Child Health Hospital, 76 Qingnian Road, Weifang, 261011, People’s Republic of China

    Jian Gao

  4. Department of Internal Medicine, University of Cincinnati, Cincinnati, OH, USA

    Joel Gabre

  5. Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert B. Sabin Way, Room 4255 MSB, Cincinnati, OH, 45267-0576, USA

    Yana Zavros Ph.D.

Authors
  1. Nina Bertaux-Skeirik
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  2. Jomaris Centeno
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  3. Jian Gao
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  4. Joel Gabre
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  5. Yana Zavros Ph.D.
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Corresponding author

Correspondence to Yana Zavros Ph.D. .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2016 Springer Science+Business Media New York

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Bertaux-Skeirik, N., Centeno, J., Gao, J., Gabre, J., Zavros, Y. (2016). Oncogenic Transformation of Human-Derived Gastric Organoids. In: Turksen, K. (eds) Organoids. Methods in Molecular Biology, vol 1576. Humana, New York, NY. https://doi.org/10.1007/7651_2016_4

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  • DOI: https://doi.org/10.1007/7651_2016_4

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7616-4

  • Online ISBN: 978-1-4939-7617-1

  • eBook Packages: Springer Protocols

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