Abstract
Skeletal muscle stem cells are satellite cells that play crucial roles in tissue repair and regeneration after muscle injury. Accumulating evidence indicates that satellite cells are genetically and functionally heterogeneous, even within the same muscle. A small population of satellite cells possesses “stemness” and exhibits the remarkable ability to regenerate through robust self-renewal when transplanted into a regenerating muscle niche. In contrast, not all satellite cells self-renew. For example, some cells are committed myogenic progenitors that immediately undergo myogenic differentiation with minimal cell division after activation. Recent studies illuminate the cellular and molecular characteristics of the functional heterogeneity among satellite cells. To evaluate heterogeneity and stem cell dynamics, here we describe methods to conduct a clonal analysis of satellite cells and to visualize a slowly dividing cell population.
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Acknowledgements
The authors thank Daiki Seko for the image shown in Fig. 1a. This work was supported by the Special Coordination Funds for Promoting Science and Technology from the Japan Science and Technology Agency (JST), a Grant-in-Aid for Challenging Exploratory Research (Research Project Number 25560338) and a Grant-in-Aid for Young Scientists A (Research Project Number 15H05368) from the Japan Society for the Promotion of Science (JSPS), and the Ichiro Kanehara Foundation.
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Kitajima, Y., Ogawa, S., Ono, Y. (2016). Visualizing the Functional Heterogeneity of Muscle Stem Cells. In: Turksen, K. (eds) Stem Cell Heterogeneity. Methods in Molecular Biology, vol 1516. Humana Press, New York, NY. https://doi.org/10.1007/7651_2016_349
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DOI: https://doi.org/10.1007/7651_2016_349
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6550-2
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