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Isolation and Characterization of Cancer Stem Cells of the Non-Small-Cell Lung Cancer (A549) Cell Line

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Stem Cell Heterogeneity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1516))

Abstract

Cancer is a major health problem worldwide. The failure of current treatments to completely eradicate cancer cells often leads to cancer recurrence and dissemination. Studies have suggested that tumor growth and spread are driven by a minority of cancer cells that exhibit characteristics similar to those of normal stem cells, thus these cells are called cancer stem cells (CSCs). CSCs are believed to play an important role in initiating and promoting cancer. CSCs are resistant to currently available cancer therapies, and understanding the mechanisms that control the growth of CSCs might have great implications for cancer therapy. Cancer cells are consist of heterogeneous population of cells, thus methods of identification, isolation, and characterisation of CSCs are fundamental to obtain a pure CSC populations. Therefore, this chapter describes in detail a method for isolating and characterizing a pure population of CSCs from heterogeneous population of cancer cells and CSCs based on specific cell surface markers.

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Authors and Affiliations

  1. Regenerative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, Penang, 13200, Malaysia

    Noor Hanis Abu Halim, Norashikin Zakaria, Nazilah Abdul Satar & Badrul Hisham Yahaya

Authors
  1. Noor Hanis Abu Halim
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  2. Norashikin Zakaria
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  3. Nazilah Abdul Satar
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  4. Badrul Hisham Yahaya
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Corresponding author

Correspondence to Badrul Hisham Yahaya .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2016 Springer Science+Business Media New York

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Halim, N.H.A., Zakaria, N., Satar, N.A., Yahaya, B.H. (2016). Isolation and Characterization of Cancer Stem Cells of the Non-Small-Cell Lung Cancer (A549) Cell Line. In: Turksen, K. (eds) Stem Cell Heterogeneity. Methods in Molecular Biology, vol 1516. Humana Press, New York, NY. https://doi.org/10.1007/7651_2016_326

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  • DOI: https://doi.org/10.1007/7651_2016_326

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6549-6

  • Online ISBN: 978-1-4939-6550-2

  • eBook Packages: Springer Protocols

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