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Development of a Bladder Bioreactor for Tissue Engineering in Urology

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Bioreactors in Stem Cell Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1502))

Abstract

A urinary bladder bioreactor was constructed to replicate physiological bladder dynamics. A cyclical low-delivery pressure regulator mimicked filling pressures of the human bladder. Cell growth was evaluated by culturing human urothelial cells (UCs) on porcine extracellular matrix scaffolds (ECMs) in the bioreactor and in static growth conditions for 5 consecutive days. UC proliferation was compared with quantitative viability indicators and by fluorescent markers for intracellular esterase activity and plasma membrane integrity. Scaffold integrity was characterized with scanning electron microscopy and 4,6-diamidino-2-phenylindole staining.

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References

  1. Biers SM, Venn SN, Greenwell TJ (2012) The past, present and future of augmentation cystoplasty. BJU Int 109:1280–1293

    Article  PubMed  Google Scholar 

  2. Davis NF, Mooney R, Callanan A et al (2011) Augmentation cystoplasty and extracellular matrix scaffolds: an ex vivo comparative study with autogenous detubularised ileum. PLoS One 6(5):1–7

    Article  CAS  Google Scholar 

  3. Flood HD, Malhotra SJ, O’Connell HE et al (1995) Long-term results and complications using augmentation cystoplasty in reconstructive urology. Neurourol Urodyn 14(4):297–309

    Article  PubMed  CAS  Google Scholar 

  4. Crapo PM, Gilbert TW, Badylak SF (2011) An overview of tissue and whole organ decellularization processes. Biomaterials 32:3233–3243

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  5. Davis NF, McGuire BB, Callanan A et al (2010) Xenogenic extracellular matrices as potential biomaterials for interposition grafting in urological surgery. J Urol 184(6):2246–2253

    Article  PubMed  CAS  Google Scholar 

  6. Zhang Y, McNeill E, Tian H et al (2008) Urine derived cells are a potential source for urological tissue reconstruction. J Urol 180(5):2226–2233

    Article  PubMed  CAS  Google Scholar 

  7. Atala A, Bauer SB, Soker S et al (2006) Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet 367:1241–1246

    Article  PubMed  Google Scholar 

  8. Davis NF, Mooney R, Piterina AV et al (2011) Construction and evaluation of urinary bladder bioreactor for urologic tissue-engineering purposes. Urology 78(4):954–960

    Article  PubMed  Google Scholar 

  9. Wallis MC, Yeger H, Cartwright L et al (2008) Feasibility study of a novel urinary bladder bioreactor. Tissue Eng Part A 14(3):339–348

    Article  PubMed  CAS  Google Scholar 

  10. Davis NF, Callanan A, McGuire BB et al (2011) Evaluation of viability and proliferative activity of human urothelial cells cultured onto xenogenic tissue-engineered extracellular matrices. Urology 77(4):1007.e1–1007.e7

    Article  Google Scholar 

  11. Callanan A, Davis NF, Walsh MT et al (2012) Mechanical characterisation of unidirectional and cross-directional multilayered urinary bladder matrix (UBM) scaffolds. Med Eng Phys 34(9):1368–1374

    Article  PubMed  Google Scholar 

  12. Shaikh FM, O’Brien TP, Callanan A et al (2010) New pulsatile hydrostatic pressure bioreactor for vascular tissue-engineered constructs. Artif Organs 34(2):153–158

    Article  PubMed  Google Scholar 

  13. Brown B, Lindberg K, Reing J et al (2006) The basement membrane component of biologic scaffolds derived from extracellular matrix. Tissue Eng 12(3):519–526

    Article  PubMed  CAS  Google Scholar 

  14. Gilbert TW, Sellaro TL, Badylak SF (2006) Decellularization of tissues and organs. Biomaterials 27:3675–3683

    PubMed  CAS  Google Scholar 

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Correspondence to Niall F. Davis .

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Davis, N.F., Callanan, A. (2015). Development of a Bladder Bioreactor for Tissue Engineering in Urology. In: Turksen, K. (eds) Bioreactors in Stem Cell Biology. Methods in Molecular Biology, vol 1502. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_309

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  • DOI: https://doi.org/10.1007/7651_2015_309

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6476-5

  • Online ISBN: 978-1-4939-6478-9

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