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Type-1 Diabetes pp 119–125Cite as

Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes

Part of the Methods in Molecular Biology book series (MIMB,volume 1433)

Abstract

MHCI-peptide tetramer staining is an important technique in order to identify antigen-specific T cells within a heterogeneous cell population. The reagents may be used to isolate antigen-specific T cells and can help identify their role in disease. Here, we describe how to make tetramer from peptide:MHC monomers together with a protocol for staining antigen-specific cell populations with advice on generating a complementary antibody phenotyping panel.

Keywords:

  • MHCI-peptide tetramer staining
  • Antigen-specific
  • T cell receptor
  • Flow cytometry

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  • DOI: 10.1007/7651_2015_295
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Acknowledgement

This work was supported by a Medical Research Council (grant number G0901155) to FSW and a Diabetes UK PhD studentship to JAP. We acknowledge the NIH Tetramer Core Facility (contract HHSN272201300006C) for provision of MHC | peptide tetramers.

Author information

Authors and Affiliations

  1. Institute of Molecular and Experimental Medicine, Cardiff School of Medicine, Cardiff University, Tenovus Building, Heath Park, CF14 4XN, UK

    James A. Pearson & F. Susan Wong

Authors
  1. James A. Pearson
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  2. F. Susan Wong
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Corresponding author

Correspondence to F. Susan Wong .

Editor information

Editors and Affiliations

  1. Diabetes and Metabolism Unit, School of Clinical Sciences, Southmead Hospital, University of Bristol, Bristol, United Kingdom

    Kathleen M. Gillespie

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Pearson, J.A., Wong, F.S. (2015). Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes. In: Gillespie, K. (eds) Type-1 Diabetes. Methods in Molecular Biology, vol 1433. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_295

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  • DOI: https://doi.org/10.1007/7651_2015_295

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3641-0

  • Online ISBN: 978-1-4939-3643-4

  • eBook Packages: Springer Protocols