Abstract
Rapid amplification of cDNA ends (RACE) is a technique that was developed to swiftly and efficiently amplify full-length RNA molecules in which the terminal ends have not been characterized. Current usage of this procedure has been more focused on sequencing and characterizing RNA 5′ and 3′ untranslated regions. Herein is described an adapted RACE protocol to amplify bacterial RNA transcripts.
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References
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Miller, E. (2015). Rapid Amplification of cDNA Ends for RNA Transcript Sequencing in Staphylococcus. In: Bose, J. (eds) The Genetic Manipulation of Staphylococci. Methods in Molecular Biology, vol 1373. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_282
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DOI: https://doi.org/10.1007/7651_2015_282
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3157-6
Online ISBN: 978-1-4939-3158-3
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