Abstract
Induced pluripotent stem cells (iPSCs) offer great promise as tools for basic biomedical research, disease modeling, and drug screening. In this chapter, we describe the generation of patient-specific, transgene-free iPSCs from skin biopsies and peripheral blood mononuclear cells through electroporation of episomal vectors and growth under two different culture conditions. The resulting iPSC lines are characterized with respect to pluripotency marker expression through immunostaining, tested for transgene integration by PCR, and assayed for differentiation capacity via teratoma formation.
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Acknowledgements
Work in our laboratory is supported by a TUBITAK 3501 grant (212T095), EMBO installation grant 2543, and FP7 Marie Curie CIG 333918 CMR.
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Fidan, K., Ebrahimi, A., Çağlayan, Ö.H., Özçimen, B., Önder, T.T. (2015). Transgene-Free Disease-Specific iPSC Generation from Fibroblasts and Peripheral Blood Mononuclear Cells. In: Nagy, A., Turksen, K. (eds) Patient-Specific Induced Pluripotent Stem Cell Models. Methods in Molecular Biology, vol 1353. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_278
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DOI: https://doi.org/10.1007/7651_2015_278
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