Abstract
Molecular cloning is a cornerstone of modern biology laboratories. However, traditional cloning can be time-consuming and problematic. We outline herein a method that utilizes the endogenous gap repair system of yeast cells to clone and assemble DNA constructs. This system is simple, cheap, and requires minimal reagents. It can be used for the assembly of both simple (single DNA fragments) and complex (multiple DNA fragments) constructs into plasmids.
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Mashruwala, A.A., Boyd, J.M. (2015). De Novo Assembly of Plasmids Using Yeast Recombinational Cloning. In: Bose, J. (eds) The Genetic Manipulation of Staphylococci. Methods in Molecular Biology, vol 1373. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_275
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DOI: https://doi.org/10.1007/7651_2015_275
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3157-6
Online ISBN: 978-1-4939-3158-3
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