De Novo Assembly of Plasmids Using Yeast Recombinational Cloning

  • Ameya A. Mashruwala
  • Jeffrey M. BoydEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1373)


Molecular cloning is a cornerstone of modern biology laboratories. However, traditional cloning can be time-consuming and problematic. We outline herein a method that utilizes the endogenous gap repair system of yeast cells to clone and assemble DNA constructs. This system is simple, cheap, and requires minimal reagents. It can be used for the assembly of both simple (single DNA fragments) and complex (multiple DNA fragments) constructs into plasmids.


Yeast Molecular cloning Staphylococcus Homologous recombination Genetics Molecular biology 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Biochemistry and MicrobiologyRutgers UniversityNew BrunswickUSA

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