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Population Epigenetics pp 1–15Cite as

Library Construction for High-Throughput Mobile Element Identification and Genotyping

Part of the Methods in Molecular Biology book series (MIMB,volume 1589)

Abstract

Mobile genetic elements are discrete DNA elements that can move around and copy themselves in a genome. As a ubiquitous component of the genome, mobile elements contribute to both genetic and epigenetic variation. Therefore, it is important to determine the genome-wide distribution of mobile elements. Here we present a targeted high-throughput sequencing protocol called Mobile Element Scanning (ME-Scan) for genome-wide mobile element detection. We will describe oligonucleotides design, sequencing library construction, and computational analysis for the ME-Scan protocol.

Keywords:

  • Mobile element
  • ME-Scan
  • High-throughput sequencing
  • Population diversity
  • Polymorphism

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Acknowledgement

The authors declare no competing financial interests. We thank Drs. David Ray and Roy Platt for their valuable comments. This study was supported by grants from the National Institutes of Health (R00HG005846).

Author information

Authors and Affiliations

  1. Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, NJ, USA

    Hongseok Ha, Nan Wang & Jinchuan Xing

  2. Human Genetic Institute of New Jersey, Rutgers, the State University of New Jersey, Piscataway, NJ, USA

    Hongseok Ha, Nan Wang & Jinchuan Xing

Authors
  1. Hongseok Ha
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  2. Nan Wang
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  3. Jinchuan Xing
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Corresponding author

Correspondence to Jinchuan Xing .

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Editors and Affiliations

  1. Rowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, Aberdeenshire, United Kingdom

    Paul Haggarty

  2. Rowett Institute of Nutrition and Health, University of Aberdeen , Bucksburn, Aberdeenshire, United Kingdom

    Kristina Harrison

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Ha, H., Wang, N., Xing, J. (2015). Library Construction for High-Throughput Mobile Element Identification and Genotyping. In: Haggarty, P., Harrison, K. (eds) Population Epigenetics. Methods in Molecular Biology, vol 1589. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_265

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  • DOI: https://doi.org/10.1007/7651_2015_265

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6901-2

  • Online ISBN: 978-1-4939-6903-6

  • eBook Packages: Springer Protocols

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