Skip to main content
SpringerLink
Log in

Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System

  • Protocol
  • First Online:
Multiple Sclerosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1304))

Abstract

Experimental autoimmune encephalomyelitis (EAE) is an animal model commonly used to investigate the inflammatory response in organ-specific autoimmunity and a model of the early immune responses of multiple sclerosis.

This protocol outlines the methods used for the processing of peripheral immune tissues, the spleen and draining lymph nodes, as well as the site of inflammation, the central nervous system (CNS), for analyzing immune cell phenotype and function during murine EAE.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. McGeachy MJ, Stephens LA et al (2005) Natural recovery and protection from autoimmune encephalomyelitis: contribution of CD4+ CD25+ regulatory cells within the central nervous system. J Immunol 175(5):3025–3032

    Article  CAS  PubMed  Google Scholar 

  2. Mildner A, Mack M et al (2009) CCR2+ Ly-6Chi monocytes are crucial for the effector phase of autoimmunity in the central nervous system. Brain 132(Pt 9):2487–2500

    Article  PubMed  Google Scholar 

  3. Butovsky O, Siddiqui S et al (2012) Modulating inflammatory monocytes with a unique microRNA gene signature ameliorates murine ALS. J Clin Invest 122(9):3063–3087

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Vainchtein ID, Vinet J et al (2014) In acute experimental autoimmune encephalomyelitis, infiltrating macrophages are immune activated, whereas microglia remain immune suppressed. Glia 62:1724–1735. doi:10.1002/glia.22711

    Article  CAS  PubMed  Google Scholar 

  5. Gautier EL, Shay T et al (2012) Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages. Nat Immunol 13(11):1118–1128

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. King IL, Dickendesher TL et al (2009) Circulating Ly-6C+ myeloid precursors migrate to the CNS and play a pathogenic role during autoimmune demyelinating disease. Blood 113(14):3190–3197

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Drutman SB, Kendall JC et al (2012) Inflammatory spleen monocytes can upregulate CD11c expression without converting into dendritic cells. J Immunol 188(8):3603–3610

    Article  CAS  PubMed  Google Scholar 

  8. Hume DA (2008) Macrophages as APC and the dendritic cell myth. J Immunol 181(9):5829–5835

    Article  CAS  PubMed  Google Scholar 

  9. Schreiner B, Bailey SL et al (2008) PD-1 ligands expressed on myeloid-derived APC in the CNS regulate T-cell responses in EAE. Eur J Immunol 38(10):2706–2717

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgements

Work in the authors’ laboratory was supported by grants from the UK Medical Research Council and the Wellcome Trust.

Author information

Authors and Affiliations

  1. MRC Centre for Inflammation Research, Queen’s Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK

    Darryl G. Turner, Melanie D. Leech, Richard A. O’Connor & Stephen M. Anderton

  2. Centre for Multiple Sclerosis Research, Queen’s Medical Research Institute, University of Edinburgh, Edinburgh, EH16 4TJ, UK

    Darryl G. Turner, Melanie D. Leech, Richard A. O’Connor & Stephen M. Anderton

Authors
  1. Darryl G. Turner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Melanie D. Leech
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Richard A. O’Connor
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Stephen M. Anderton
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Stephen M. Anderton .

Editor information

Editors and Affiliations

  1. University of Regensburg, Regensburg, Germany

    Robert Weissert

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media New York

About this protocol

Cite this protocol

Turner, D.G., Leech, M.D., O’Connor, R.A., Anderton, S.M. (2015). Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System. In: Weissert, R. (eds) Multiple Sclerosis. Methods in Molecular Biology, vol 1304. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_222

Download citation

  • DOI: https://doi.org/10.1007/7651_2015_222

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2629-9

  • Online ISBN: 978-1-4939-2630-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation