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Using Oct4:MerCreMer Lineage Tracing to Monitor Endogenous Oct4 Expression During the Reprogramming of Fibroblasts into Induced Pluripotent Stem Cells (iPSCs)

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1357)

Abstract

The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) using a combination of defined transcription factors has become one of the most widely used techniques in stem cell biology. A critical, early event in iPSC reprogramming is the induction of the endogenous transcription factor network that maintains pluripotency in iPSCs. Here we describe using a transgenic, conditional Oct4-Cre construct to investigate the spatial and temporal induction of endogenous Oct4 expression during the reprogramming of mouse fibroblasts into iPS cells.

Keywords:

Oct4 Oct4-Cre Induced pluripotent stem cell iPSC Reprogramming Lineage tracing 
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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, John Radcliffe HospitalUniversity of OxfordOxfordUK
  2. 2.Stem Cell InstituteUniversity of MinnesotaMinneapolisUSA

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