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Stem Cell Renewal and Cell-Cell Communication pp 195–200Cite as

Electrophysiological Recordings from Neuroepithelial Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1212))

Abstract

During embryonic development, neuroepithelial cells act as neural stem cells by renewing themselves. These cells are tightly interconnected and make contact with the basement membrane of the neuroepithelium. Under such circumstances, intracellular recording with a fine-tipped microelectrode is a successful method to study the electrophysiological properties of the neuroepithelial stem cell. This chapter describes the detailed techniques of intracellular recording from neuroepithelial stem cells.

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References

  1. Yamashita M (2012) Ion channel activities in neural stem cells of the neuroepithelium. Stem Cells Int 2012:847670. doi:10.1155/2012/247670

    Article  Google Scholar 

  2. Yamashita M (2013) From neuroepithelial cells to neurons: changes in the physiological properties of neuroepithelial stem cells. Arch Biochem Biophys 534:64–70

    Article  CAS  PubMed  Google Scholar 

  3. Warner AE (1973) The electrical properties of the ectoderm in the amphibian embryo during induction and early development of the nervous system. J Physiol 235:267–286

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Yamashita M, Fukuda Y (1993) Calcium channels and GABA receptors in the early embryonic chick retina. J Neurobiol 24:1600–1614

    Article  CAS  PubMed  Google Scholar 

  5. Yamashita M (2008) Synchronous Ca2+ oscillation emerges from voltage fluctuations of Ca2+ stores. FEBS J 275:4022–4032

    Article  CAS  PubMed  Google Scholar 

  6. Brown KT, Flaming DG (1986) Advanced micropipette techniques for cell physiology. In: Smith AD (ed) IBRO handbook series: methods in the neurosciences, vol 9. Wiley, New York, NY

    Google Scholar 

  7. Mobbs P et al (1994) Techniques for dye injection and cell labelling. In: Ogden D (ed) Microelectrode techniques, 2nd edn, The Plymouth workshop handbook. The company of Biologists Limited, London, pp 361–387

    Google Scholar 

  8. Yamashita M (2013) Electric axon guidance in embryonic retina: galvanotropism revisited. Biochem Biophys Res Commun 431:280–283

    Article  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Centre for Medical Science, International University of Health and Welfare, Kitakanemaru 2600-1, Ohtawara, 324-8501, Japan

    Masayuki Yamashita

Authors
  1. Masayuki Yamashita
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Corresponding author

Correspondence to Masayuki Yamashita .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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Yamashita, M. (2014). Electrophysiological Recordings from Neuroepithelial Stem Cells. In: Turksen, K. (eds) Stem Cell Renewal and Cell-Cell Communication. Methods in Molecular Biology, vol 1212. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_96

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  • DOI: https://doi.org/10.1007/7651_2014_96

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2589-6

  • Online ISBN: 978-1-4939-2590-2

  • eBook Packages: Springer Protocols

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