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Fabrication of Biofunctionalized, Cell-Laden Macroporous 3D PEG Hydrogels as Bone Marrow Analogs for the Cultivation of Human Hematopoietic Stem and Progenitor Cells

  • Lisa Rödling
  • Annamarija Raic
  • Cornelia Lee-Thedieck
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1202)

Abstract

In vitro proliferation of hematopoietic stem cells (HSCs) is yet an unresolved challenge. Found in the bone marrow, HSCs can undergo self-renewing cell division and thereby multiply. Recapitulation of the bone marrow environment in order to provide the required signals for their expansion is a promising approach.

Here, we describe a technique to produce biofunctionalized, macroporous poly(ethylene glycol) diacrylate (PEGDA) hydrogels that mimic the spongy 3D architecture of trabecular bones, which host the red, blood-forming bone marrow. After seeding these scaffolds with cells, they can be used as simplified bone marrow analogs for the cultivation of HSCs. This method can easily be conducted with standard laboratory chemicals and equipment. The 3D hydrogels are produced via salt leaching and biofunctionalization of the material is achieved by co-polymerizing the PEGDA with an RGD peptide. Finally, cell seeding and retrieval are described.

Keywords

Hematopoietic stem and progenitor cells Biomimetic material 3D scaffolds Bone marrow analog Stem cell niche 

Notes

Acknowledgements

This work was supported by the BioInterfaces programme of the Helmholtz-Association, the “Brigitte Schlieben-Lange-Programm”, the “Käthe und Josef Klinz-Stiftung”, and the BMBF NanoMatFutur programme (FKZ: 13N12968).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Lisa Rödling
    • 1
  • Annamarija Raic
    • 2
  • Cornelia Lee-Thedieck
    • 1
  1. 1.Institute of Functional InterfacesKarlsruhe Institute of Technology (KIT)KarlsruheGermany
  2. 2.RWTH Aachen UniversityAachenGermany

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