Abstract
Human pluripotent stem cells (hPSCs), including human embryonic stem cells and human-induced pluripotent stem cells, are a renewable cell source for a wide range of applications in regenerative medicine and useful tools for human disease modeling and drug discovery. For these purposes, large numbers of high-quality cells are essential. Recently, we showed that a biological substrate, recombinant E8 fragments of laminin isoforms, sustains long-term self-renewal of hPSCs in defined, xeno-free medium with dissociated single-cell passaging. Here, we describe a modified culture system with similar performance to efficiently expand hPSCs under defined, xeno-free conditions using a non-biological synthetic substrate.
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Acknowledgments
I thank Ms. Fumi Kashigi and Mari Hamao for their valuable technical assistance and the members of the laboratories of N. Nakatsuji for insightful discussions. This work was supported by a Grant-in-Aid for Scientific Research and partially supported by the New Energy and Industrial Technology Development Organization (NEDO) of Japan.
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Kawase, E. (2014). Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate. In: Turksen, K. (eds) Human Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 1307. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_82
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DOI: https://doi.org/10.1007/7651_2014_82
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