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Human Embryonic Stem Cell Protocols pp 281–287Cite as

Microgrooved Surface Modulates Neuron Differentiation in Human Embryonic Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1307))

Abstract

Stem cell-based therapies have drawn intensive attention in the neuronal regenerative fields. Several studies have revealed that stem cells can serve as an inexhaustible source for neurons for transplantation therapies. However, generation of neurons and directionality has not yet been fully investigated. Herein, we investigate the mechanical ramifications of surface topography on human embryonic cell differentiation. Microgrooved surfaces with various pitches were applied to modulate the neuron differentiation. Our protocol showed that neuron differentiation increased as grove pitch decreased. The results indicated that 2 μm microgrooves can improve neuron growth by ~1.7-fold. Our results indicate the importance of mechanotransduction on neuronal differentiation and highlight the feasibility of manipulating the neuronal differentiation with surface topography, providing new perspectives for accommodating clinical transplantation.

An erratum to this chapter is available at 10.1007/7651_2014_86

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Acknowledgments

We would like to acknowledge Muscular Dystrophy Association (MDA), UC Merced GRC Summer Fellowships, and Chang Gung University CMRP D1C0031 for support and funding. We would also like to thank Philip Lee and Catherine Le for their assistance.

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Author notes

    Authors and Affiliations

    1. Bioengineering, University of California, Merced, CA, USA

      David Lu, Chi-Shuo Chen, Sushant Soni, Clarence Le, Eric Y.-T. Chen & Wei-Chun Chin

    2. Department of Electronic Engineering, Chang Gung University, Taoyuan, Taiwan

      Chao-Sung Lai & Eric Y.-T. Chen

    3. School of Nature Sciences, University of California, Merced, CA, USA

      Taranze Lam

    4. Department of Neurology, The John Hopkins University School of Medicine, Baltimore, MD, USA

      Thien Nguyen

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    1. David Lu
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    2. Chi-Shuo Chen
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    3. Chao-Sung Lai
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    4. Sushant Soni
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    5. Taranze Lam
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    6. Clarence Le
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    7. Eric Y.-T. Chen
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    9. Wei-Chun Chin
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    Corresponding authors

    Correspondence to Chao-Sung Lai or Wei-Chun Chin .

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    Editors and Affiliations

    1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

      Kursad Turksen

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    Lu, D. et al. (2014). Microgrooved Surface Modulates Neuron Differentiation in Human Embryonic Stem Cells. In: Turksen, K. (eds) Human Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 1307. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_81

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    • DOI: https://doi.org/10.1007/7651_2014_81

    • Published:

    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-2667-1

    • Online ISBN: 978-1-4939-2668-8

    • eBook Packages: Springer Protocols

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