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Generation of a Transposon Mutant Library in Staphylococcus aureus and Staphylococcus epidermidis Using bursa aurealis

Part of the Methods in Molecular Biology book series (MIMB,volume 1373)

Abstract

Transposon mutagenesis is a genetic process that involves the random insertion of transposons into a genome resulting in the disruption of function of the genes in which they insert. Identification of the insertion sites through DNA sequencing allows for the identification of the genes disrupted and the creation of “libraries” containing a collection of mutants in which a large number of the nonessential genes have been disrupted. These mutant libraries have been a great resource for investigators to understand the various biological functions of individual genes, including those involved in metabolism, antibiotic susceptibility, and pathogenesis. Here, we describe the detailed methodologies for constructing a sequence defined transposon mutant library in both Staphylococcus aureus and S. epidermidis using the mariner-based transposon, bursa aurealis.

Keywords

  • Bursa aurealis
  • Transposon
  • Staphylococcus aureus
  • Staphylococcus epidermidis

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  • DOI: 10.1007/7651_2014_189
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Correspondence to Kenneth W. Bayles .

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Yajjala, V.K., Widhelm, T.J., Endres, J.L., Fey, P.D., Bayles, K.W. (2014). Generation of a Transposon Mutant Library in Staphylococcus aureus and Staphylococcus epidermidis Using bursa aurealis . In: Bose, J. (eds) The Genetic Manipulation of Staphylococci. Methods in Molecular Biology, vol 1373. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_189

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  • DOI: https://doi.org/10.1007/7651_2014_189

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3157-6

  • Online ISBN: 978-1-4939-3158-3

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