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Patient-Specific Induced Pluripotent Stem Cell Models pp 25–44Cite as

Generation and Characterization of Patient-Specific Induced Pluripotent Stem Cell for Disease Modeling

Part of the Methods in Molecular Biology book series (MIMB,volume 1353)

Abstract

One major hurdle to the development of effective treatments to many diseases is the lack of suitable human model systems. The ability to reprogram human somatic cells to induced pluripotent stem cells (iPSC) offers an excellent opportunity to generate human disease models with primary cells. Currently, several methods to generate iPSC lines exist, and iPSC can be generated from various tissue sources including skin fibroblasts, blood, hair follicles, dental tissue, and urine. In this chapter we describe the generation and characterization of iPSC from blood or fibroblast on a routine base and focus on the integration-free methodologies.

Keywords

  • iPSC
  • Integration-free
  • Disease modeling
  • Parkinson’s disease

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Author information

Authors and Affiliations

  1. Buck Institute for Research on Aging, 8001 Redwood Blvd., Novato, CA, 94945, USA

    Renuka Sivapatham & Xianmin Zeng Ph.D.

Authors
  1. Renuka Sivapatham
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  2. Xianmin Zeng Ph.D.
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Corresponding author

Correspondence to Xianmin Zeng Ph.D. .

Editor information

Editors and Affiliations

  1. Lunenfeld-Tanenbaum Research Institute, Toronto, Ontario, Canada

    Andras Nagy

  2. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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Sivapatham, R., Zeng, X. (2014). Generation and Characterization of Patient-Specific Induced Pluripotent Stem Cell for Disease Modeling. In: Nagy, A., Turksen, K. (eds) Patient-Specific Induced Pluripotent Stem Cell Models. Methods in Molecular Biology, vol 1353. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_157

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  • DOI: https://doi.org/10.1007/7651_2014_157

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3033-3

  • Online ISBN: 978-1-4939-3034-0

  • eBook Packages: Springer Protocols

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