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Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs

Part of the Methods in Molecular Biology book series (MIMB,volume 1283)

Abstract

The clinical use of human induced pluripotent stem cells (hiPSCs) and the development of patients-specific gene and cell therapies rely on the development of fast, reliable, and integration-free methods of derivation of pluripotent stem cells from somatic tissues. Here we describe an integration-free protocol for the rapid derivation of hiPSCs from dermal fibroblasts using modified mRNAs. This method is inexpensive, highly efficient, and makes use of reagents that are xeno-free and chemically defined and can therefore be adopted by any Good Manufacturing Practice (GMP) facility.

Keywords:

  • Xeno-free hiPSCs
  • GMP
  • Clinical use
  • Modified mRNA
  • Regenerative medicine
  • Cell therapy
  • Gene therapy

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  • DOI: 10.1007/7651_2014_124
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Correspondence to Vittorio Sebastiano .

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Durruthy, J.D., Sebastiano, V. (2014). Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs. In: Turksen, K. (eds) Stem Cells and Good Manufacturing Practices. Methods in Molecular Biology, vol 1283. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_124

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  • DOI: https://doi.org/10.1007/7651_2014_124

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2434-9

  • Online ISBN: 978-1-4939-2435-6

  • eBook Packages: Springer Protocols