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The Suspension Culture of Undifferentiated Human Pluripotent Stem Cells Using Spinner Flasks

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Stem Cells and Good Manufacturing Practices

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1283))

Abstract

Suspension cell culture systems with superior scalability, controllability, and monitoring options are an attractive alternative to static adherent culture methods for expansion and production of human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent cells (iPSCs). In this chapter, we describe a scalable suspension culture system using serum-free, feeder-free, matrix-free, and defined culture conditions with spinner flasks for hPSC maintenance and expansion. This suspension culture system provides an efficient and GMP-compatible process for large-scale manufacture of hPSCs.

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Author information

Authors and Affiliations

  1. Center for Applied Technology Development, Beckman Research Institute of City of Hope, 1500 E. Duarte Road, Duarte, CA, 91010, USA

    Vincent C. Chen & Larry A. Couture

Authors
  1. Vincent C. Chen
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  2. Larry A. Couture
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Corresponding author

Correspondence to Larry A. Couture .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2014 Springer Science+Business Media New York

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Chen, V.C., Couture, L.A. (2014). The Suspension Culture of Undifferentiated Human Pluripotent Stem Cells Using Spinner Flasks. In: Turksen, K. (eds) Stem Cells and Good Manufacturing Practices. Methods in Molecular Biology, vol 1283. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_118

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  • DOI: https://doi.org/10.1007/7651_2014_118

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2434-9

  • Online ISBN: 978-1-4939-2435-6

  • eBook Packages: Springer Protocols

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