Abstract
From a perspective of manufacturer, procurement of bone marrow aspirates for isolation of mesenchymal stromal/stem cells (MSC) is challenging. The MSC isolated from adult donors have lower proliferation capacity than the cells isolated form young donors of pediatric age. To obtain more MSC from young healthy donors for allogeneic therapy on multiple patients, umbilical cord (UC) seems to be the best alternative. Here, we describe an easy, cost-effective and reproducible protocol of isolation of the MSC from Wharton’s Jelly (WJ) in UC.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Gottipamula S, Muttigi MS, Chaansa S, Ashwin KM, Priya N, Kolkundkar U, Sundar Raj S, Majumdar AS, Seetharam RN (2013) Large-scale expansion of pre-isolated bone marrow mesenchymal stromal cells in serum-free conditions. J Tissue Eng Regen Med. [Epub ahead of print]
Patrikoski M, Juntunen M, Boucher S, Campbell A, Vemuri MC, Mannerstrom B, Miettinen S (2013) Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells. Stem Cell Res Ther 4:27
Chase LG, Yang S, Zachar V, Yang Z, Lakshmipathy U, Bradford J, Boucher SE, Vemuri MC (2012) Development and characterization of a clinically compliant xeno-free culture medium in good manufacturing practice for human multipotent mesenchymal stem cells. Stem Cells Transl Med 1:750–758
Fong CY, Richards M, Manasi N, Biswas A, Bongso A (2007) Comparative growth behaviour and characterization of stem cells from human Wharton's jelly. Reprod Biomed Online 15:708–718
Fong CY, Subramanian A, Biswas A, Gauthaman K, Srikanth P, Hande MP, Bongso A (2010) Derivation efficiency, cell proliferation, frozen-thaw survival, stem-cell properties, and differentiation of human Wharton’s jelly stem cells. Reprod Biomed Online 21:391–401
Pereira WC, Khushnooma I, Madkaikar M, Ghosh K (2008) Reproducible methodology for the isolation of mesenchymal stem cells from human umbilical cord and its potential for cardiomyocyte generation. J Tissue Eng Regen Med 2:394–399
Weiss ML, Anderson C, Medicetty S, Seshareddy KB, Weiss RJ, Werff IV, Troyer D, McIntosh KR (2008) Immune properties of human umbilical cord Wharton’s jelly-derived cells. Stem Cells 26:2865–2874
Kadam SS, Tiwari S, Bhonde RR (2009) Simultaneous isolation of vascular endothelial cells and mesenchymal stem cells from the human umbilical cord. In Vitro Cell Dev Biol Anim 45:23–27
Conconi MT, Di Liddo R, Tommasini M, Calore C, Parnigotto PP (2011) Phenotype and differentiation potential of stromal populations obtained from various zones of human umbilical cord: an overview. Open Tissue Eng Regen Med J 4:6–20
Mark P, Kleinsorge M, Gaebel R, Lux CA, Toelk A, Pittermann E, David R, Steinhoff G, Ma N (2013) Human mesenchymal stem cells display reduced expression of CD105 after culture in serum-free medium. Stem Cells Int 2013:698076
Ilic D, Almeida EA, Schlaepfer DD, Dazin P, Aizawa S, Damsky CH (1998) Extracellular matrix survival signals transduced by focal adhesion kinase suppress p53-mediated apoptosis. J Cell Biol 143:547–560
Almeida EA, Ilic D, Han Q, Hauck CR, Jin F, Kawakatsu H, Schlaepfer DD, Damsky CH (2000) Matrix survival signaling: from fibronectin via focal adhesion kinase to c-Jun NH(2)-terminal kinase. J Cell Biol 149:741–754
Acknowledgments
The study was supported by the studentship to H.B. from Saudi Arabia government.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Badraiq, H., Devito, L., Ilic, D. (2014). Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions. In: Turksen, K. (eds) Stem Cells and Good Manufacturing Practices. Methods in Molecular Biology, vol 1283. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_116
Download citation
DOI: https://doi.org/10.1007/7651_2014_116
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2434-9
Online ISBN: 978-1-4939-2435-6
eBook Packages: Springer Protocols