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Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions

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Stem Cells and Good Manufacturing Practices

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1283))

Abstract

From a perspective of manufacturer, procurement of bone marrow aspirates for isolation of mesenchymal stromal/stem cells (MSC) is challenging. The MSC isolated from adult donors have lower proliferation capacity than the cells isolated form young donors of pediatric age. To obtain more MSC from young healthy donors for allogeneic therapy on multiple patients, umbilical cord (UC) seems to be the best alternative. Here, we describe an easy, cost-effective and reproducible protocol of isolation of the MSC from Wharton’s Jelly (WJ) in UC.

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Acknowledgments

The study was supported by the studentship to H.B. from Saudi Arabia government.

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Correspondence to Dusko Ilic .

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© 2014 Springer Science+Business Media New York

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Badraiq, H., Devito, L., Ilic, D. (2014). Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions. In: Turksen, K. (eds) Stem Cells and Good Manufacturing Practices. Methods in Molecular Biology, vol 1283. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_116

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  • DOI: https://doi.org/10.1007/7651_2014_116

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2434-9

  • Online ISBN: 978-1-4939-2435-6

  • eBook Packages: Springer Protocols

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