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Stem Cells and Good Manufacturing Practices pp 137–145Cite as

Purification of Human Induced Pluripotent Stem Cell-Derived Neural Precursors Using Magnetic Activated Cell Sorting

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1283))

Abstract

Neural precursor (NP) cells derived from human induced pluripotent stem cells (hiPSCs), and their neuronal progeny, will play an important role in disease modeling, drug screening tests, central nervous system development studies, and may even become valuable for regenerative medicine treatments. Nonetheless, it is challenging to obtain homogeneous and synchronously differentiated NP populations from hiPSCs, and after neural commitment many pluripotent stem cells remain in the differentiated cultures. Here, we describe an efficient and simple protocol to differentiate hiPSC-derived NPs in 12 days, and we include a final purification stage where Tra-1-60+ pluripotent stem cells (PSCs) are removed using magnetic activated cell sorting (MACS), leaving the NP population nearly free of PSCs.

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Acknowledgements

This work was funded by Fundação para a Ciência e a Tecnologia, Portugal (Project PTDC/EBB-BIO/122504/2010). Gonçalo M.C. Rodrigues, Tiago G. Fernandes, and Carlos A.V. Rodrigues were supported by fellowships from Fundação para a Ciência e a Tecnologia, Portugal (SFRH/BD/89374/2012, SFRH/BPD/86316/2012, and SFRH/BPD/82056/2011, respectively). We acknowledge Professor Oliver Brustle for providing the hiPSC cell line.

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Authors and Affiliations

  1. Department of Bioengineering and IBB – Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001, Lisbon, Portugal

    Gonçalo M. C. Rodrigues, Tiago G. Fernandes, Carlos A. V. Rodrigues, Joaquim M. S. Cabral & Maria Margarida Diogo

Authors
  1. Gonçalo M. C. Rodrigues
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  2. Tiago G. Fernandes
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  3. Carlos A. V. Rodrigues
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  4. Joaquim M. S. Cabral
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  5. Maria Margarida Diogo
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Corresponding author

Correspondence to Maria Margarida Diogo .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2014 Springer Science+Business Media New York

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Rodrigues, G.M.C., Fernandes, T.G., Rodrigues, C.A.V., Cabral, J.M.S., Diogo, M.M. (2014). Purification of Human Induced Pluripotent Stem Cell-Derived Neural Precursors Using Magnetic Activated Cell Sorting. In: Turksen, K. (eds) Stem Cells and Good Manufacturing Practices. Methods in Molecular Biology, vol 1283. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_115

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  • DOI: https://doi.org/10.1007/7651_2014_115

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2434-9

  • Online ISBN: 978-1-4939-2435-6

  • eBook Packages: Springer Protocols

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