Mimicking Bone Microenvironment for Directing Adipose Tissue-Derived Mesenchymal Stem Cells into Osteogenic Differentiation

  • ZuFu Lu
  • Seyed-Iman Roohani-Esfahani
  • Hala ZreiqatEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1202)


Adipose tissue-derived mesenchymal stem cells (ASCs) have become an increasingly interested cell source for the scientists in the fields of stem cell biology and regenerative medicine. ASCs have already been used in a number of clinical trials, and some successful outcomes have been reported in bone tissue regeneration. Here we describe the protocols which mimic the factors in bone healing microenvironment, including inflammation burst, osteoblasts, and bone biomimetic scaffolds to direct ASCs into osteogenic differentiation.


Adipose tissue-derived mesenchymal stem cells Bone microenvironment Osteogenic differentiation TNF-α Osteoblasts Biomimetic scaffolds 


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • ZuFu Lu
    • 2
  • Seyed-Iman Roohani-Esfahani
    • 2
  • Hala Zreiqat
    • 1
    Email author
  1. 1.Biomaterials and Tissue Engineering Research UnitSchool of AMME, The University of SydneySydneyAustralia
  2. 2.Biomaterials and Tissue Engineering Research UnitSchool of AMME, The University of SydneySydneyAustralia

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