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Mimicking Bone Microenvironment for Directing Adipose Tissue-Derived Mesenchymal Stem Cells into Osteogenic Differentiation

  • ZuFu Lu
  • Seyed-Iman Roohani-Esfahani
  • Hala Zreiqat
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1202)

Abstract

Adipose tissue-derived mesenchymal stem cells (ASCs) have become an increasingly interested cell source for the scientists in the fields of stem cell biology and regenerative medicine. ASCs have already been used in a number of clinical trials, and some successful outcomes have been reported in bone tissue regeneration. Here we describe the protocols which mimic the factors in bone healing microenvironment, including inflammation burst, osteoblasts, and bone biomimetic scaffolds to direct ASCs into osteogenic differentiation.

Keywords

Adipose tissue-derived mesenchymal stem cells Bone microenvironment Osteogenic differentiation TNF-α Osteoblasts Biomimetic scaffolds 

References

  1. 1.
    Zuk PA, Zhu M, Ashjian P et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13:4279–4295PubMedCentralPubMedCrossRefGoogle Scholar
  2. 2.
    Seda Tigli R, Ghosh S, Laha MM et al (2009) Comparative chondrogenesis of human cell sources in 3D scaffolds. J Tissue Eng Regen Med 3:348–360PubMedCentralPubMedCrossRefGoogle Scholar
  3. 3.
    Levi B, Longaker MT (2011) Concise review: adipose-derived stromal cells for skeletal regenerative medicine. Stem Cells 29:576–582PubMedCentralPubMedCrossRefGoogle Scholar
  4. 4.
    Mauney JR, Nguyen T, Gillen K et al (2007) Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds. Biomaterials 28:5280–5290PubMedCentralPubMedCrossRefGoogle Scholar
  5. 5.
    Wilson A, Butler PE, Seifalian AM (2011) Adipose-derived stem cells for clinical applications: a review. Cell Prolif 44:86–98PubMedCrossRefGoogle Scholar
  6. 6.
    Szpalski C, Barbaro M, Sagebin F et al (2012) Bone tissue engineering: current strategies and techniques–part II: cell types. Tissue Eng Part B Rev 18:258–269PubMedCrossRefGoogle Scholar
  7. 7.
    Giannoni P, Lazzarini E, Ceseracciu L et al (2012) Design and characterization of a tissue-engineered bilayer scaffold for osteochondral tissue repair. J Tissue Eng Regen Med. doi: 10.1002/term.1651 PubMedGoogle Scholar
  8. 8.
    Hwang NS, Varghese S, Lee HJ et al (2013) Biomaterials directed in vivo osteogenic differentiation of mesenchymal cells derived from human embryonic stem cells. Tissue Eng Part A 16(15–16):1723–1732. doi: 10.1089/ten.tea.2013.0064 CrossRefGoogle Scholar
  9. 9.
    Lu Z, Roohani-Esfahani SI, Kwok PC et al (2011) Osteoblasts on rod shaped hydroxyapatite nanoparticles incorporated PCL film provide an optimal osteogenic niche for stem cell differentiation. Tissue Eng Part A 17:1651–1661PubMedCrossRefGoogle Scholar
  10. 10.
    Lu Z, Roohani-Esfahani SI, Wang G et al (2012) Bone biomimetic microenvironment induces osteogenic differentiation of adipose tissue-derived mesenchymal stem cells. Nanomedicine 8:507–515PubMedCrossRefGoogle Scholar
  11. 11.
    Lu Z, Wang G, Dunstan CR et al (2013) Activation and promotion of adipose stem cells by tumour necrosis factor-alpha preconditioning for bone regeneration. J Cell Physiol 228:1737–1744PubMedCrossRefGoogle Scholar
  12. 12.
    Almeida CR, Vasconcelos DP, Goncalves RM et al (2012) Enhanced mesenchymal stromal cell recruitment via natural killer cells by incorporation of inflammatory signals in biomaterials. J R Soc Interface 9:261–271PubMedCentralPubMedCrossRefGoogle Scholar
  13. 13.
    Mountziaris PM, Mikos AG (2008) Modulation of the inflammatory response for enhanced bone tissue regeneration. Tissue Eng Part B Rev 14:179–186PubMedCentralPubMedCrossRefGoogle Scholar
  14. 14.
    Bae SE, Bhang SH, Kim BS et al (2012) Self-assembled extracellular macromolecular matrices and their different osteogenic potential with preosteoblasts and rat bone marrow mesenchymal stromal cells. Biomacromolecules 13:2811–2820PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • ZuFu Lu
    • 2
  • Seyed-Iman Roohani-Esfahani
    • 2
  • Hala Zreiqat
    • 1
  1. 1.Biomaterials and Tissue Engineering Research UnitSchool of AMME, The University of SydneySydneyAustralia
  2. 2.Biomaterials and Tissue Engineering Research UnitSchool of AMME, The University of SydneySydneyAustralia

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